Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay

Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrh...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Darren Y. J., Ashcroft, Melinda M., Chow, Eric P. F., Sait, Michelle, De Petra, Vesna, Tschaepe, Marlene, Lange, Sigrid, Taiaroa, George, Bradshaw, Catriona S., Whiley, David M., Fairley, Christopher K., Howden, Benjamin P., Chen, Marcus Y., Pasricha, Shivani, Williamson, Deborah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091848/
https://www.ncbi.nlm.nih.gov/pubmed/33658263
http://dx.doi.org/10.1128/JCM.00089-21
_version_ 1783687557480972288
author Lee, Darren Y. J.
Ashcroft, Melinda M.
Chow, Eric P. F.
Sait, Michelle
De Petra, Vesna
Tschaepe, Marlene
Lange, Sigrid
Taiaroa, George
Bradshaw, Catriona S.
Whiley, David M.
Fairley, Christopher K.
Howden, Benjamin P.
Chen, Marcus Y.
Pasricha, Shivani
Williamson, Deborah A.
author_facet Lee, Darren Y. J.
Ashcroft, Melinda M.
Chow, Eric P. F.
Sait, Michelle
De Petra, Vesna
Tschaepe, Marlene
Lange, Sigrid
Taiaroa, George
Bradshaw, Catriona S.
Whiley, David M.
Fairley, Christopher K.
Howden, Benjamin P.
Chen, Marcus Y.
Pasricha, Shivani
Williamson, Deborah A.
author_sort Lee, Darren Y. J.
collection PubMed
description Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.
format Online
Article
Text
id pubmed-8091848
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-80918482021-10-20 Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay Lee, Darren Y. J. Ashcroft, Melinda M. Chow, Eric P. F. Sait, Michelle De Petra, Vesna Tschaepe, Marlene Lange, Sigrid Taiaroa, George Bradshaw, Catriona S. Whiley, David M. Fairley, Christopher K. Howden, Benjamin P. Chen, Marcus Y. Pasricha, Shivani Williamson, Deborah A. J Clin Microbiol Bacteriology Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows. American Society for Microbiology 2021-04-20 /pmc/articles/PMC8091848/ /pubmed/33658263 http://dx.doi.org/10.1128/JCM.00089-21 Text en Copyright © 2021 Lee et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bacteriology
Lee, Darren Y. J.
Ashcroft, Melinda M.
Chow, Eric P. F.
Sait, Michelle
De Petra, Vesna
Tschaepe, Marlene
Lange, Sigrid
Taiaroa, George
Bradshaw, Catriona S.
Whiley, David M.
Fairley, Christopher K.
Howden, Benjamin P.
Chen, Marcus Y.
Pasricha, Shivani
Williamson, Deborah A.
Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title_full Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title_fullStr Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title_full_unstemmed Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title_short Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay
title_sort reflex detection of ciprofloxacin resistance in neisseria gonorrhoeae by use of the speedx resistanceplus gc assay
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091848/
https://www.ncbi.nlm.nih.gov/pubmed/33658263
http://dx.doi.org/10.1128/JCM.00089-21
work_keys_str_mv AT leedarrenyj reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT ashcroftmelindam reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT chowericpf reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT saitmichelle reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT depetravesna reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT tschaepemarlene reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT langesigrid reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT taiaroageorge reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT bradshawcatrionas reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT whileydavidm reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT fairleychristopherk reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT howdenbenjaminp reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT chenmarcusy reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT pasrichashivani reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay
AT williamsondeboraha reflexdetectionofciprofloxacinresistanceinneisseriagonorrhoeaebyuseofthespeedxresistanceplusgcassay

Ejemplares similares