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Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication
We interrogated at nucleotide resolution the spatiotemporal order of chromatin changes that occur immediately following a site-specific double-strand break (DSB) upstream of the PHO5 locus and its subsequent repair by nonhomologous end joining (NHEJ). We observed the immediate eviction of a nucleoso...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092003/ https://www.ncbi.nlm.nih.gov/pubmed/33811083 http://dx.doi.org/10.1101/gr.271155.120 |
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author | Tripuraneni, Vinay Memisoglu, Gonen MacAlpine, Heather K. Tran, Trung Q. Zhu, Wei Hartemink, Alexander J. Haber, James E. MacAlpine, David M. |
author_facet | Tripuraneni, Vinay Memisoglu, Gonen MacAlpine, Heather K. Tran, Trung Q. Zhu, Wei Hartemink, Alexander J. Haber, James E. MacAlpine, David M. |
author_sort | Tripuraneni, Vinay |
collection | PubMed |
description | We interrogated at nucleotide resolution the spatiotemporal order of chromatin changes that occur immediately following a site-specific double-strand break (DSB) upstream of the PHO5 locus and its subsequent repair by nonhomologous end joining (NHEJ). We observed the immediate eviction of a nucleosome flanking the break and the repositioning of adjacent nucleosomes away from the break. These early chromatin events were independent of the end-processing Mre11-Rad50-Xrs2 (MRX) complex and preceded the MRX-dependent broad eviction of histones and DNA end-resectioning that extends up to ∼8 kb away from the break. We also examined the temporal dynamics of NHEJ-mediated repair in a G1-arrested population. Concomitant with DSB repair by NHEJ, we observed the redeposition and precise repositioning of nucleosomes at their originally occupied positions. This re-establishment of the prelesion chromatin landscape suggests that a DNA replication-independent mechanism exists to preserve epigenome organization following DSB repair. |
format | Online Article Text |
id | pubmed-8092003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80920032021-11-01 Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication Tripuraneni, Vinay Memisoglu, Gonen MacAlpine, Heather K. Tran, Trung Q. Zhu, Wei Hartemink, Alexander J. Haber, James E. MacAlpine, David M. Genome Res Research We interrogated at nucleotide resolution the spatiotemporal order of chromatin changes that occur immediately following a site-specific double-strand break (DSB) upstream of the PHO5 locus and its subsequent repair by nonhomologous end joining (NHEJ). We observed the immediate eviction of a nucleosome flanking the break and the repositioning of adjacent nucleosomes away from the break. These early chromatin events were independent of the end-processing Mre11-Rad50-Xrs2 (MRX) complex and preceded the MRX-dependent broad eviction of histones and DNA end-resectioning that extends up to ∼8 kb away from the break. We also examined the temporal dynamics of NHEJ-mediated repair in a G1-arrested population. Concomitant with DSB repair by NHEJ, we observed the redeposition and precise repositioning of nucleosomes at their originally occupied positions. This re-establishment of the prelesion chromatin landscape suggests that a DNA replication-independent mechanism exists to preserve epigenome organization following DSB repair. Cold Spring Harbor Laboratory Press 2021-05 /pmc/articles/PMC8092003/ /pubmed/33811083 http://dx.doi.org/10.1101/gr.271155.120 Text en © 2021 Tripuraneni et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see https://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Research Tripuraneni, Vinay Memisoglu, Gonen MacAlpine, Heather K. Tran, Trung Q. Zhu, Wei Hartemink, Alexander J. Haber, James E. MacAlpine, David M. Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title | Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title_full | Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title_fullStr | Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title_full_unstemmed | Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title_short | Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication |
title_sort | local nucleosome dynamics and eviction following a double-strand break are reversible by nhej-mediated repair in the absence of dna replication |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092003/ https://www.ncbi.nlm.nih.gov/pubmed/33811083 http://dx.doi.org/10.1101/gr.271155.120 |
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