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Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics

Cryptococcus neoformans causes deadly mycosis in immunocompromised individuals. Macrophages are key cells fighting against microbes. Extracellular vesicles (EVs) are cell-to-cell communication mediators. The roles of EVs from infected host cells in the interaction with Cryptococcus remain uninvestig...

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Autores principales: Zhang, Lei, Zhang, Keming, Li, Hang, Coelho, Carolina, de Souza Gonçalves, Diego, Fu, Man Shun, Li, Xinhua, Nakayasu, Ernesto S., Kim, Young-Mo, Liao, Wanqing, Pan, Weihua, Casadevall, Arturo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092229/
https://www.ncbi.nlm.nih.gov/pubmed/33785616
http://dx.doi.org/10.1128/mBio.00279-21
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author Zhang, Lei
Zhang, Keming
Li, Hang
Coelho, Carolina
de Souza Gonçalves, Diego
Fu, Man Shun
Li, Xinhua
Nakayasu, Ernesto S.
Kim, Young-Mo
Liao, Wanqing
Pan, Weihua
Casadevall, Arturo
author_facet Zhang, Lei
Zhang, Keming
Li, Hang
Coelho, Carolina
de Souza Gonçalves, Diego
Fu, Man Shun
Li, Xinhua
Nakayasu, Ernesto S.
Kim, Young-Mo
Liao, Wanqing
Pan, Weihua
Casadevall, Arturo
author_sort Zhang, Lei
collection PubMed
description Cryptococcus neoformans causes deadly mycosis in immunocompromised individuals. Macrophages are key cells fighting against microbes. Extracellular vesicles (EVs) are cell-to-cell communication mediators. The roles of EVs from infected host cells in the interaction with Cryptococcus remain uninvestigated. Here, EVs from viable C. neoformans-infected macrophages reduced fungal burdens but led to shorter survival of infected mice. In vitro, EVs induced naive macrophages to an inflammatory phenotype. Transcriptome analysis showed that EVs from viable C. neoformans-infected macrophages activated immune-related pathways, including p53 in naive human and murine macrophages. Conserved analysis demonstrated that basic cell biological processes, including cell cycle and division, were activated by infection-derived EVs from both murine and human infected macrophages. Combined proteomics, lipidomics, and metabolomics of EVs from infected macrophages showed regulation of pathways such as extracellular matrix (ECM) receptors and phosphatidylcholine. This form of intermacrophage communication could serve to prepare cells at more distant sites of infection to resist C. neoformans infection.
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spelling pubmed-80922292021-05-04 Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics Zhang, Lei Zhang, Keming Li, Hang Coelho, Carolina de Souza Gonçalves, Diego Fu, Man Shun Li, Xinhua Nakayasu, Ernesto S. Kim, Young-Mo Liao, Wanqing Pan, Weihua Casadevall, Arturo mBio Research Article Cryptococcus neoformans causes deadly mycosis in immunocompromised individuals. Macrophages are key cells fighting against microbes. Extracellular vesicles (EVs) are cell-to-cell communication mediators. The roles of EVs from infected host cells in the interaction with Cryptococcus remain uninvestigated. Here, EVs from viable C. neoformans-infected macrophages reduced fungal burdens but led to shorter survival of infected mice. In vitro, EVs induced naive macrophages to an inflammatory phenotype. Transcriptome analysis showed that EVs from viable C. neoformans-infected macrophages activated immune-related pathways, including p53 in naive human and murine macrophages. Conserved analysis demonstrated that basic cell biological processes, including cell cycle and division, were activated by infection-derived EVs from both murine and human infected macrophages. Combined proteomics, lipidomics, and metabolomics of EVs from infected macrophages showed regulation of pathways such as extracellular matrix (ECM) receptors and phosphatidylcholine. This form of intermacrophage communication could serve to prepare cells at more distant sites of infection to resist C. neoformans infection. American Society for Microbiology 2021-03-30 /pmc/articles/PMC8092229/ /pubmed/33785616 http://dx.doi.org/10.1128/mBio.00279-21 Text en Copyright © 2021 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhang, Lei
Zhang, Keming
Li, Hang
Coelho, Carolina
de Souza Gonçalves, Diego
Fu, Man Shun
Li, Xinhua
Nakayasu, Ernesto S.
Kim, Young-Mo
Liao, Wanqing
Pan, Weihua
Casadevall, Arturo
Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title_full Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title_fullStr Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title_full_unstemmed Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title_short Cryptococcus neoformans-Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
title_sort cryptococcus neoformans-infected macrophages release proinflammatory extracellular vesicles: insight into their components by multi-omics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092229/
https://www.ncbi.nlm.nih.gov/pubmed/33785616
http://dx.doi.org/10.1128/mBio.00279-21
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