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ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia
SARS-CoV-2 infection causing the COVID-19 pandemic calls for immediate interventions to avoid viral transmission, disease progression, and subsequent excessive inflammation and tissue destruction. Primary normal human bronchial epithelial cells are among the first targets of SARS-CoV-2 infection. He...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092264/ https://www.ncbi.nlm.nih.gov/pubmed/33906927 http://dx.doi.org/10.1128/mBio.00904-21 |
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author | Posch, W. Vosper, J. Zaderer, V. Noureen, A. Constant, S. Bellmann-Weiler, R. Lass-Flörl, C. Wilflingseder, D. |
author_facet | Posch, W. Vosper, J. Zaderer, V. Noureen, A. Constant, S. Bellmann-Weiler, R. Lass-Flörl, C. Wilflingseder, D. |
author_sort | Posch, W. |
collection | PubMed |
description | SARS-CoV-2 infection causing the COVID-19 pandemic calls for immediate interventions to avoid viral transmission, disease progression, and subsequent excessive inflammation and tissue destruction. Primary normal human bronchial epithelial cells are among the first targets of SARS-CoV-2 infection. Here, we show that ColdZyme medical device mouth spray efficiently protected against virus entry, excessive inflammation, and tissue damage. Applying ColdZyme to fully differentiated, polarized human epithelium cultured at an air-liquid interphase (ALI) completely blocked binding of SARS-CoV-2 and increased local complement activation mediated by the virus as well as productive infection of the tissue model. While SARS-CoV-2 infection resulted in exaggerated intracellular complement activation immediately following infection and a drop in transepithelial resistance, these parameters were bypassed by single pretreatment of the tissues with ColdZyme mouth spray. Crucially, our study highlights the importance of testing already evaluated and safe drugs such as ColdZyme mouth spray for maintaining epithelial integrity and hindering SARS-CoV-2 entry within standardized three-dimensional (3D) in vitro models mimicking the in vivo human airway epithelium. |
format | Online Article Text |
id | pubmed-8092264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-80922642021-05-04 ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia Posch, W. Vosper, J. Zaderer, V. Noureen, A. Constant, S. Bellmann-Weiler, R. Lass-Flörl, C. Wilflingseder, D. mBio Research Article SARS-CoV-2 infection causing the COVID-19 pandemic calls for immediate interventions to avoid viral transmission, disease progression, and subsequent excessive inflammation and tissue destruction. Primary normal human bronchial epithelial cells are among the first targets of SARS-CoV-2 infection. Here, we show that ColdZyme medical device mouth spray efficiently protected against virus entry, excessive inflammation, and tissue damage. Applying ColdZyme to fully differentiated, polarized human epithelium cultured at an air-liquid interphase (ALI) completely blocked binding of SARS-CoV-2 and increased local complement activation mediated by the virus as well as productive infection of the tissue model. While SARS-CoV-2 infection resulted in exaggerated intracellular complement activation immediately following infection and a drop in transepithelial resistance, these parameters were bypassed by single pretreatment of the tissues with ColdZyme mouth spray. Crucially, our study highlights the importance of testing already evaluated and safe drugs such as ColdZyme mouth spray for maintaining epithelial integrity and hindering SARS-CoV-2 entry within standardized three-dimensional (3D) in vitro models mimicking the in vivo human airway epithelium. American Society for Microbiology 2021-04-27 /pmc/articles/PMC8092264/ /pubmed/33906927 http://dx.doi.org/10.1128/mBio.00904-21 Text en Copyright © 2021 Posch et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Posch, W. Vosper, J. Zaderer, V. Noureen, A. Constant, S. Bellmann-Weiler, R. Lass-Flörl, C. Wilflingseder, D. ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title | ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title_full | ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title_fullStr | ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title_full_unstemmed | ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title_short | ColdZyme Maintains Integrity in SARS-CoV-2-Infected Airway Epithelia |
title_sort | coldzyme maintains integrity in sars-cov-2-infected airway epithelia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092264/ https://www.ncbi.nlm.nih.gov/pubmed/33906927 http://dx.doi.org/10.1128/mBio.00904-21 |
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