Cargando…
Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi
The fungi are an enormously successful eukaryotic lineage that has colonized every aerobic habitat on Earth. This spectacular expansion is reflected in the dynamism and diversity of the fungal cell wall, a matrix of polysaccharides and glycoproteins pivotal to fungal life history strategies and a ma...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092308/ https://www.ncbi.nlm.nih.gov/pubmed/33849982 http://dx.doi.org/10.1128/mBio.03540-20 |
_version_ | 1783687635729907712 |
---|---|
author | Schwerdt, Julian Qiu, Hao Shirley, Neil Little, Alan Bulone, Vincent |
author_facet | Schwerdt, Julian Qiu, Hao Shirley, Neil Little, Alan Bulone, Vincent |
author_sort | Schwerdt, Julian |
collection | PubMed |
description | The fungi are an enormously successful eukaryotic lineage that has colonized every aerobic habitat on Earth. This spectacular expansion is reflected in the dynamism and diversity of the fungal cell wall, a matrix of polysaccharides and glycoproteins pivotal to fungal life history strategies and a major target in the development of antifungal compounds. Cell wall polysaccharides are typically synthesized by Leloir glycosyltransferases, enzymes that are notoriously difficult to characterize, but their nucleotide-sugar substrates are well known and provide the opportunity to inspect the monosaccharides available for incorporation into cell wall polysaccharides and glycoproteins. In this work, we have used phylogenomic analyses of the enzymatic pathways that synthesize and interconvert nucleotide-sugars to predict potential cell wall monosaccharide composition across 491 fungal taxa. The results show a complex evolutionary history of these cell wall enzyme pathways and, by association, of the fungal cell wall. In particular, we see a significant reduction in monosaccharide diversity during fungal evolution, most notably in the colonization of terrestrial habitats. However, monosaccharide distribution is also shown to be varied across later-diverging fungal lineages. |
format | Online Article Text |
id | pubmed-8092308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-80923082021-05-04 Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi Schwerdt, Julian Qiu, Hao Shirley, Neil Little, Alan Bulone, Vincent mBio Research Article The fungi are an enormously successful eukaryotic lineage that has colonized every aerobic habitat on Earth. This spectacular expansion is reflected in the dynamism and diversity of the fungal cell wall, a matrix of polysaccharides and glycoproteins pivotal to fungal life history strategies and a major target in the development of antifungal compounds. Cell wall polysaccharides are typically synthesized by Leloir glycosyltransferases, enzymes that are notoriously difficult to characterize, but their nucleotide-sugar substrates are well known and provide the opportunity to inspect the monosaccharides available for incorporation into cell wall polysaccharides and glycoproteins. In this work, we have used phylogenomic analyses of the enzymatic pathways that synthesize and interconvert nucleotide-sugars to predict potential cell wall monosaccharide composition across 491 fungal taxa. The results show a complex evolutionary history of these cell wall enzyme pathways and, by association, of the fungal cell wall. In particular, we see a significant reduction in monosaccharide diversity during fungal evolution, most notably in the colonization of terrestrial habitats. However, monosaccharide distribution is also shown to be varied across later-diverging fungal lineages. American Society for Microbiology 2021-04-13 /pmc/articles/PMC8092308/ /pubmed/33849982 http://dx.doi.org/10.1128/mBio.03540-20 Text en Copyright © 2021 Schwerdt et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Schwerdt, Julian Qiu, Hao Shirley, Neil Little, Alan Bulone, Vincent Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title | Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title_full | Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title_fullStr | Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title_full_unstemmed | Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title_short | Phylogenomic Analyses of Nucleotide-Sugar Biosynthetic and Interconverting Enzymes Illuminate Cell Wall Composition in Fungi |
title_sort | phylogenomic analyses of nucleotide-sugar biosynthetic and interconverting enzymes illuminate cell wall composition in fungi |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092308/ https://www.ncbi.nlm.nih.gov/pubmed/33849982 http://dx.doi.org/10.1128/mBio.03540-20 |
work_keys_str_mv | AT schwerdtjulian phylogenomicanalysesofnucleotidesugarbiosyntheticandinterconvertingenzymesilluminatecellwallcompositioninfungi AT qiuhao phylogenomicanalysesofnucleotidesugarbiosyntheticandinterconvertingenzymesilluminatecellwallcompositioninfungi AT shirleyneil phylogenomicanalysesofnucleotidesugarbiosyntheticandinterconvertingenzymesilluminatecellwallcompositioninfungi AT littlealan phylogenomicanalysesofnucleotidesugarbiosyntheticandinterconvertingenzymesilluminatecellwallcompositioninfungi AT bulonevincent phylogenomicanalysesofnucleotidesugarbiosyntheticandinterconvertingenzymesilluminatecellwallcompositioninfungi |