Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-...

Descripción completa

Detalles Bibliográficos
Autores principales: Abd Algaffar, S. O., Verbon, A., van de Sande, W. W. J., Khalid, S. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092548/
https://www.ncbi.nlm.nih.gov/pubmed/33318015
http://dx.doi.org/10.1128/AAC.01338-20
Descripción
Sumario:We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC(50), 0.016 µg/ml) followed by voriconazole (MIC(50), 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.

Ejemplares similares