Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-...

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Autores principales: Abd Algaffar, S. O., Verbon, A., van de Sande, W. W. J., Khalid, S. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092548/
https://www.ncbi.nlm.nih.gov/pubmed/33318015
http://dx.doi.org/10.1128/AAC.01338-20
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author Abd Algaffar, S. O.
Verbon, A.
van de Sande, W. W. J.
Khalid, S. A.
author_facet Abd Algaffar, S. O.
Verbon, A.
van de Sande, W. W. J.
Khalid, S. A.
author_sort Abd Algaffar, S. O.
collection PubMed
description We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC(50), 0.016 µg/ml) followed by voriconazole (MIC(50), 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.
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spelling pubmed-80925482021-05-05 Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis Abd Algaffar, S. O. Verbon, A. van de Sande, W. W. J. Khalid, S. A. Antimicrob Agents Chemother Susceptibility We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC(50), 0.016 µg/ml) followed by voriconazole (MIC(50), 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective. American Society for Microbiology 2021-02-17 /pmc/articles/PMC8092548/ /pubmed/33318015 http://dx.doi.org/10.1128/AAC.01338-20 Text en Copyright © 2021 Abd Algaffar et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Susceptibility
Abd Algaffar, S. O.
Verbon, A.
van de Sande, W. W. J.
Khalid, S. A.
Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title_full Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title_fullStr Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title_full_unstemmed Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title_short Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis
title_sort development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis
topic Susceptibility
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092548/
https://www.ncbi.nlm.nih.gov/pubmed/33318015
http://dx.doi.org/10.1128/AAC.01338-20
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