A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an ort...

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Autores principales: Rauch, Jennifer N., Valois, Eric, Solley, Sabrina C., Braig, Friederike, Lach, Ryan S., Audouard, Morgane, Ponce-Rojas, Jose Carlos, Costello, Michael S., Baxter, Naomi J., Kosik, Kenneth S., Arias, Carolina, Acosta-Alvear, Diego, Wilson, Maxwell Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092748/
https://www.ncbi.nlm.nih.gov/pubmed/33478979
http://dx.doi.org/10.1128/JCM.02402-20
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author Rauch, Jennifer N.
Valois, Eric
Solley, Sabrina C.
Braig, Friederike
Lach, Ryan S.
Audouard, Morgane
Ponce-Rojas, Jose Carlos
Costello, Michael S.
Baxter, Naomi J.
Kosik, Kenneth S.
Arias, Carolina
Acosta-Alvear, Diego
Wilson, Maxwell Z.
author_facet Rauch, Jennifer N.
Valois, Eric
Solley, Sabrina C.
Braig, Friederike
Lach, Ryan S.
Audouard, Morgane
Ponce-Rojas, Jose Carlos
Costello, Michael S.
Baxter, Naomi J.
Kosik, Kenneth S.
Arias, Carolina
Acosta-Alvear, Diego
Wilson, Maxwell Z.
author_sort Rauch, Jennifer N.
collection PubMed
description The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance.
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spelling pubmed-80927482021-05-21 A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material Rauch, Jennifer N. Valois, Eric Solley, Sabrina C. Braig, Friederike Lach, Ryan S. Audouard, Morgane Ponce-Rojas, Jose Carlos Costello, Michael S. Baxter, Naomi J. Kosik, Kenneth S. Arias, Carolina Acosta-Alvear, Diego Wilson, Maxwell Z. J Clin Microbiol Virology The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance. American Society for Microbiology 2021-03-19 /pmc/articles/PMC8092748/ /pubmed/33478979 http://dx.doi.org/10.1128/JCM.02402-20 Text en Copyright © 2021 Rauch et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . https://doi.org/10.1128/ASMCopyrightv2This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Virology
Rauch, Jennifer N.
Valois, Eric
Solley, Sabrina C.
Braig, Friederike
Lach, Ryan S.
Audouard, Morgane
Ponce-Rojas, Jose Carlos
Costello, Michael S.
Baxter, Naomi J.
Kosik, Kenneth S.
Arias, Carolina
Acosta-Alvear, Diego
Wilson, Maxwell Z.
A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title_full A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title_fullStr A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title_full_unstemmed A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title_short A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
title_sort scalable, easy-to-deploy protocol for cas13-based detection of sars-cov-2 genetic material
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092748/
https://www.ncbi.nlm.nih.gov/pubmed/33478979
http://dx.doi.org/10.1128/JCM.02402-20
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