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Salivary testing of COVID-19: evaluation of serological testing following positive salivary results
BACKGROUND: Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase ch...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093594/ https://www.ncbi.nlm.nih.gov/pubmed/33947347 http://dx.doi.org/10.1186/s12879-021-06108-5 |
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author | Caulley, Lisa Shaw, Julie Corsten, Martin Hua, Nadia Angel, Jonathan B. Poliquin, Guillaume Whelan, Jonathan Antonation, Kym Johnson-Obaseki, Stephanie |
author_facet | Caulley, Lisa Shaw, Julie Corsten, Martin Hua, Nadia Angel, Jonathan B. Poliquin, Guillaume Whelan, Jonathan Antonation, Kym Johnson-Obaseki, Stephanie |
author_sort | Caulley, Lisa |
collection | PubMed |
description | BACKGROUND: Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate the accuracy of saliva versus swabs for COVID-19 detection and the durability of antibody response. METHODS: Venous blood samples were collected from consenting participants and the presence of serum antibodies for SARS-CoV-2 was evaluated on a large, automated immunoassay platform by the Roche anti-SARS-CoV-2 qualitative assay (Roche Diagnostics, Laval Quebec). Individuals with a serum antibody cut-off index (COI) ≥ 1.0 were considered positive. RESULTS: In asymptomatic and mildly symptomatic patients with a previously positive standard swab and/or saliva SARS-CoV-2 PCR-test, 42 demonstrated antibodies with 13 patients positive by swab alone, and 8 patients positive by saliva alone. CONCLUSIONS: Despite their status as ‘current standard’ for COVID-19 testing, these findings highlight limitations of PCR-based tests. |
format | Online Article Text |
id | pubmed-8093594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80935942021-05-04 Salivary testing of COVID-19: evaluation of serological testing following positive salivary results Caulley, Lisa Shaw, Julie Corsten, Martin Hua, Nadia Angel, Jonathan B. Poliquin, Guillaume Whelan, Jonathan Antonation, Kym Johnson-Obaseki, Stephanie BMC Infect Dis Research BACKGROUND: Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate the accuracy of saliva versus swabs for COVID-19 detection and the durability of antibody response. METHODS: Venous blood samples were collected from consenting participants and the presence of serum antibodies for SARS-CoV-2 was evaluated on a large, automated immunoassay platform by the Roche anti-SARS-CoV-2 qualitative assay (Roche Diagnostics, Laval Quebec). Individuals with a serum antibody cut-off index (COI) ≥ 1.0 were considered positive. RESULTS: In asymptomatic and mildly symptomatic patients with a previously positive standard swab and/or saliva SARS-CoV-2 PCR-test, 42 demonstrated antibodies with 13 patients positive by swab alone, and 8 patients positive by saliva alone. CONCLUSIONS: Despite their status as ‘current standard’ for COVID-19 testing, these findings highlight limitations of PCR-based tests. BioMed Central 2021-05-04 /pmc/articles/PMC8093594/ /pubmed/33947347 http://dx.doi.org/10.1186/s12879-021-06108-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Caulley, Lisa Shaw, Julie Corsten, Martin Hua, Nadia Angel, Jonathan B. Poliquin, Guillaume Whelan, Jonathan Antonation, Kym Johnson-Obaseki, Stephanie Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title | Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title_full | Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title_fullStr | Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title_full_unstemmed | Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title_short | Salivary testing of COVID-19: evaluation of serological testing following positive salivary results |
title_sort | salivary testing of covid-19: evaluation of serological testing following positive salivary results |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093594/ https://www.ncbi.nlm.nih.gov/pubmed/33947347 http://dx.doi.org/10.1186/s12879-021-06108-5 |
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