Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads (SSMBs)

Plasmid DNA (pDNA) isolation from bacterial cells is one of the most common and critical steps in molecular cloning and biomedical research. Almost all pDNA purification involves disruption of bacteria, removal of membrane lipids, proteins and genomic DNA, purification of pDNA from bulk lysate, and...

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Detalles Bibliográficos
Autores principales: Wang, Xi, Zhao, Ling, Wu, Xiaoxing, Luo, Huaxiu, Wu, Di, Zhang, Meng, Zhang, Jing, Pakvasa, Mikhail, Wagstaff, William, He, Fang, Mao, Yukun, Zhang, Yongtao, Niu, Changchun, Wu, Meng, Zhao, Xia, Wang, Hao, Huang, Linjuan, Shi, Deyao, Liu, Qing, Ni, Na, Fu, Kai, Hynes, Kelly, Strelzow, Jason, El Dafrawy, Mostafa, He, Tong-Chuan, Qi, Hongbo, Zeng, Zongyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chongqing Medical University 2020
Materias:
Full Length Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093646/
https://www.ncbi.nlm.nih.gov/pubmed/33997177
http://dx.doi.org/10.1016/j.gendis.2020.04.013

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