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The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer
Human cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093799/ https://www.ncbi.nlm.nih.gov/pubmed/33959494 http://dx.doi.org/10.3389/fonc.2021.614925 |
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author | Ye, Sisi Hu, Yuanbo Chen, Chenbin Chen, Sian Tong, Xinya Zhu, Huanbo Deng, Bo Hu, Xianjing Sun, Xiangwei Chen, Xiaodong Shi, Xinyu Gu, Ruihong Xie, Wangkai Guo, Gangqiang Xing, Dong Shen, Xian Xue, Xiangyang Shen, Shurong |
author_facet | Ye, Sisi Hu, Yuanbo Chen, Chenbin Chen, Sian Tong, Xinya Zhu, Huanbo Deng, Bo Hu, Xianjing Sun, Xiangwei Chen, Xiaodong Shi, Xinyu Gu, Ruihong Xie, Wangkai Guo, Gangqiang Xing, Dong Shen, Xian Xue, Xiangyang Shen, Shurong |
author_sort | Ye, Sisi |
collection | PubMed |
description | Human cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study was to investigate the expression of the US31 gene in GC tissue and assess its role in the occurrence and development of GC. US31 expression in 573 cancer tissues was analyzed using immunohistochemistry. Results showed that US31 was significantly associated with tumor size (P = 0.005) and distant metastasis (P < 0.001). Higher US31 expression indicated better overall survival in GC patients. Overexpression of US31 significantly inhibited the proliferation, migration, and invasion of GC cells in vitro (P < 0.05). Furthermore, expression levels of CD4, CD66b, and CD166 were positively correlated with US31, suggesting that it was involved in regulating the tumor immune microenvironment of GC. RNA sequencing, along with quantitative real-time polymerase chain reaction, confirmed that the expression of US31 promoted immune activation and secretion of inflammatory cytokines. Overall, US31 inhibited the malignant phenotype and regulated tumor immune cell infiltration in GC; these results suggest that US31 could be a potential prognostic factor for GC and may open the door for a new immunotherapy strategy. |
format | Online Article Text |
id | pubmed-8093799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80937992021-05-05 The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer Ye, Sisi Hu, Yuanbo Chen, Chenbin Chen, Sian Tong, Xinya Zhu, Huanbo Deng, Bo Hu, Xianjing Sun, Xiangwei Chen, Xiaodong Shi, Xinyu Gu, Ruihong Xie, Wangkai Guo, Gangqiang Xing, Dong Shen, Xian Xue, Xiangyang Shen, Shurong Front Oncol Oncology Human cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study was to investigate the expression of the US31 gene in GC tissue and assess its role in the occurrence and development of GC. US31 expression in 573 cancer tissues was analyzed using immunohistochemistry. Results showed that US31 was significantly associated with tumor size (P = 0.005) and distant metastasis (P < 0.001). Higher US31 expression indicated better overall survival in GC patients. Overexpression of US31 significantly inhibited the proliferation, migration, and invasion of GC cells in vitro (P < 0.05). Furthermore, expression levels of CD4, CD66b, and CD166 were positively correlated with US31, suggesting that it was involved in regulating the tumor immune microenvironment of GC. RNA sequencing, along with quantitative real-time polymerase chain reaction, confirmed that the expression of US31 promoted immune activation and secretion of inflammatory cytokines. Overall, US31 inhibited the malignant phenotype and regulated tumor immune cell infiltration in GC; these results suggest that US31 could be a potential prognostic factor for GC and may open the door for a new immunotherapy strategy. Frontiers Media S.A. 2021-04-20 /pmc/articles/PMC8093799/ /pubmed/33959494 http://dx.doi.org/10.3389/fonc.2021.614925 Text en Copyright © 2021 Ye, Hu, Chen, Chen, Tong, Zhu, Deng, Hu, Sun, Chen, Shi, Gu, Xie, Guo, Xing, Shen, Xue and Shen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Ye, Sisi Hu, Yuanbo Chen, Chenbin Chen, Sian Tong, Xinya Zhu, Huanbo Deng, Bo Hu, Xianjing Sun, Xiangwei Chen, Xiaodong Shi, Xinyu Gu, Ruihong Xie, Wangkai Guo, Gangqiang Xing, Dong Shen, Xian Xue, Xiangyang Shen, Shurong The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title_full | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title_fullStr | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title_full_unstemmed | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title_short | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
title_sort | human cytomegalovirus us31 gene predicts favorable survival and regulates the tumor microenvironment in gastric cancer |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093799/ https://www.ncbi.nlm.nih.gov/pubmed/33959494 http://dx.doi.org/10.3389/fonc.2021.614925 |
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