Cargando…

Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis

Shaoyao decoction (SYD), a classical traditional Chinese medicine formula, is effective for the treatment of inflammatory bowel disease (IBD). This study was designed to investigate the therapeutic effects of SYD on IBD and possible mechanisms. Dextran sulfate sodium (DSS, 3.5%) was used to induce c...

Descripción completa

Detalles Bibliográficos
Autores principales: Chi, Honggang, Wang, Dan, Chen, Mengting, Lin, Jiantao, Zhang, Shuhua, Yu, Fengyan, Zhou, Jun, Zheng, Xuebao, Zou, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093868/
https://www.ncbi.nlm.nih.gov/pubmed/33959000
http://dx.doi.org/10.3389/fphar.2021.524287
_version_ 1783687904313212928
author Chi, Honggang
Wang, Dan
Chen, Mengting
Lin, Jiantao
Zhang, Shuhua
Yu, Fengyan
Zhou, Jun
Zheng, Xuebao
Zou, Ying
author_facet Chi, Honggang
Wang, Dan
Chen, Mengting
Lin, Jiantao
Zhang, Shuhua
Yu, Fengyan
Zhou, Jun
Zheng, Xuebao
Zou, Ying
author_sort Chi, Honggang
collection PubMed
description Shaoyao decoction (SYD), a classical traditional Chinese medicine formula, is effective for the treatment of inflammatory bowel disease (IBD). This study was designed to investigate the therapeutic effects of SYD on IBD and possible mechanisms. Dextran sulfate sodium (DSS, 3.5%) was used to induce colitis in C57BL/6 mice. Disease phenotypes were investigated based on disease activity index (DAI), colon length, and microscopic and macroscopic scores. Additionally, the presence of proinflammatory cytokines, immune cell infiltrates, intestinal cell proliferation, apoptosis, epithelial permeability, signal transducer and activator of transcription 3 (STAT3), and nuclear factor-κB (NF-κB) signaling, as well as the intestinal mucosal barrier function, were investigated. The administration of SYD significantly ameliorated the clinical signs, suppressed the levels of proinflammatory cytokines, and reduced immune cell infiltrates into colonic tissues of DSS-induced colitis model mice. SYD also significantly reduced the DSS-induced activation of STAT3 and NF-κB signaling. Furthermore, SYD promoted epithelial integrity by regulating epithelial cell apoptosis and epithelial permeability. Finally, we demonstrated that SYD protected the intestinal barrier function by significantly regulating the mucus layer genes Muc1, Muc2, Muc4, and Tff3, as well as the epithelial barrier genes Z O -1 and Occludin. Our results indicate that SYD has a protective effect on DSS-induced colitis, which is attributable to its anti-inflammatory activity and intestinal barrier function-enhancing effects. These results provide valuable insights into the pharmacological actions of SYD for the treatment of IBD.
format Online
Article
Text
id pubmed-8093868
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80938682021-05-05 Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis Chi, Honggang Wang, Dan Chen, Mengting Lin, Jiantao Zhang, Shuhua Yu, Fengyan Zhou, Jun Zheng, Xuebao Zou, Ying Front Pharmacol Pharmacology Shaoyao decoction (SYD), a classical traditional Chinese medicine formula, is effective for the treatment of inflammatory bowel disease (IBD). This study was designed to investigate the therapeutic effects of SYD on IBD and possible mechanisms. Dextran sulfate sodium (DSS, 3.5%) was used to induce colitis in C57BL/6 mice. Disease phenotypes were investigated based on disease activity index (DAI), colon length, and microscopic and macroscopic scores. Additionally, the presence of proinflammatory cytokines, immune cell infiltrates, intestinal cell proliferation, apoptosis, epithelial permeability, signal transducer and activator of transcription 3 (STAT3), and nuclear factor-κB (NF-κB) signaling, as well as the intestinal mucosal barrier function, were investigated. The administration of SYD significantly ameliorated the clinical signs, suppressed the levels of proinflammatory cytokines, and reduced immune cell infiltrates into colonic tissues of DSS-induced colitis model mice. SYD also significantly reduced the DSS-induced activation of STAT3 and NF-κB signaling. Furthermore, SYD promoted epithelial integrity by regulating epithelial cell apoptosis and epithelial permeability. Finally, we demonstrated that SYD protected the intestinal barrier function by significantly regulating the mucus layer genes Muc1, Muc2, Muc4, and Tff3, as well as the epithelial barrier genes Z O -1 and Occludin. Our results indicate that SYD has a protective effect on DSS-induced colitis, which is attributable to its anti-inflammatory activity and intestinal barrier function-enhancing effects. These results provide valuable insights into the pharmacological actions of SYD for the treatment of IBD. Frontiers Media S.A. 2021-04-20 /pmc/articles/PMC8093868/ /pubmed/33959000 http://dx.doi.org/10.3389/fphar.2021.524287 Text en Copyright © 2021 Chi, Wang, Chen, Lin, Zhang, Yu, Zhou, Zheng and Zou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Chi, Honggang
Wang, Dan
Chen, Mengting
Lin, Jiantao
Zhang, Shuhua
Yu, Fengyan
Zhou, Jun
Zheng, Xuebao
Zou, Ying
Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title_full Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title_fullStr Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title_full_unstemmed Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title_short Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis
title_sort shaoyao decoction inhibits inflammation and improves intestinal barrier function in mice with dextran sulfate sodium-induced colitis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093868/
https://www.ncbi.nlm.nih.gov/pubmed/33959000
http://dx.doi.org/10.3389/fphar.2021.524287
work_keys_str_mv AT chihonggang shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT wangdan shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT chenmengting shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT linjiantao shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT zhangshuhua shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT yufengyan shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT zhoujun shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT zhengxuebao shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis
AT zouying shaoyaodecoctioninhibitsinflammationandimprovesintestinalbarrierfunctioninmicewithdextransulfatesodiuminducedcolitis