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Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair

This study aimed to investigate the ability of CD146(+) subset of ADSCs to repair cartilage defects. In this study, we prepared CD146(+) liposome magnetic beads (CD146(+)LMB) to isolate CD146(+)ADSCs. The cells were induced for chondrogenic differentiation and verified by cartilage‐specific mRNA and...

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Autores principales: Xie, Aiguo, Peng, Yinbo, Yao, Zuochao, Lu, Lin, Ni, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093962/
https://www.ncbi.nlm.nih.gov/pubmed/33768729
http://dx.doi.org/10.1111/jcmm.16470
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author Xie, Aiguo
Peng, Yinbo
Yao, Zuochao
Lu, Lin
Ni, Tao
author_facet Xie, Aiguo
Peng, Yinbo
Yao, Zuochao
Lu, Lin
Ni, Tao
author_sort Xie, Aiguo
collection PubMed
description This study aimed to investigate the ability of CD146(+) subset of ADSCs to repair cartilage defects. In this study, we prepared CD146(+) liposome magnetic beads (CD146(+)LMB) to isolate CD146(+)ADSCs. The cells were induced for chondrogenic differentiation and verified by cartilage‐specific mRNA and protein expression. Then a mouse model of cartilage defect was constructed and treated by filling the induced cartilage cells into the damaged joint, to evaluate the function of such cells in the cartilage microenvironment. Our results demonstrated that the CD146(+)LMBs we prepared were uniform, small and highly stable, and cell experiments showed that the CD146(+)LMB has low cytotoxicity to the ADSCs. ADSCs isolated with CD146(+)LMB were all CD146(+), CD105(+), CD166(+) and CD73(+). After chondrogenic induction, the cells showed significantly increased expression of cartilage markers Sox9, collagen Ⅱ and aggrecan at protein level and significantly increased Sox9, collagen Ⅱ and aggrecan at mRNA level, and the protein expression and mRNA expression of CD146(+)ADSCs group were higher than those of ADSCs group. The CD146(+)ADSCs group showed superior tissue repair ability than the ADSCs group and blank control group in the animal experiment, as judged by gross observation, histological observation and histological scoring. The above results proved that CD146(+)LMB can successfully isolate the CD146(+)ADSCs, and after chondrogenic induction, these cells successfully promoted repair of articular cartilage defects, which may be a new direction of tissue engineering.
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spelling pubmed-80939622021-05-10 Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair Xie, Aiguo Peng, Yinbo Yao, Zuochao Lu, Lin Ni, Tao J Cell Mol Med Original Articles This study aimed to investigate the ability of CD146(+) subset of ADSCs to repair cartilage defects. In this study, we prepared CD146(+) liposome magnetic beads (CD146(+)LMB) to isolate CD146(+)ADSCs. The cells were induced for chondrogenic differentiation and verified by cartilage‐specific mRNA and protein expression. Then a mouse model of cartilage defect was constructed and treated by filling the induced cartilage cells into the damaged joint, to evaluate the function of such cells in the cartilage microenvironment. Our results demonstrated that the CD146(+)LMBs we prepared were uniform, small and highly stable, and cell experiments showed that the CD146(+)LMB has low cytotoxicity to the ADSCs. ADSCs isolated with CD146(+)LMB were all CD146(+), CD105(+), CD166(+) and CD73(+). After chondrogenic induction, the cells showed significantly increased expression of cartilage markers Sox9, collagen Ⅱ and aggrecan at protein level and significantly increased Sox9, collagen Ⅱ and aggrecan at mRNA level, and the protein expression and mRNA expression of CD146(+)ADSCs group were higher than those of ADSCs group. The CD146(+)ADSCs group showed superior tissue repair ability than the ADSCs group and blank control group in the animal experiment, as judged by gross observation, histological observation and histological scoring. The above results proved that CD146(+)LMB can successfully isolate the CD146(+)ADSCs, and after chondrogenic induction, these cells successfully promoted repair of articular cartilage defects, which may be a new direction of tissue engineering. John Wiley and Sons Inc. 2021-03-25 2021-05 /pmc/articles/PMC8093962/ /pubmed/33768729 http://dx.doi.org/10.1111/jcmm.16470 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Xie, Aiguo
Peng, Yinbo
Yao, Zuochao
Lu, Lin
Ni, Tao
Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title_full Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title_fullStr Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title_full_unstemmed Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title_short Effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
title_sort effect of a subset of adipose‐derived stem cells isolated with liposome magnetic beads to promote cartilage repair
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093962/
https://www.ncbi.nlm.nih.gov/pubmed/33768729
http://dx.doi.org/10.1111/jcmm.16470
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