An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard e...

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Detalles Bibliográficos
Autores principales: Canibano-Fraile, Rodrigo, Boertjes, Emma, Bozhilova, Stela, Pijnappel, W.W.M. Pim, Schaaf, Gerben J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095053/
https://www.ncbi.nlm.nih.gov/pubmed/33997810
http://dx.doi.org/10.1016/j.xpro.2021.100482
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author Canibano-Fraile, Rodrigo
Boertjes, Emma
Bozhilova, Stela
Pijnappel, W.W.M. Pim
Schaaf, Gerben J.
author_facet Canibano-Fraile, Rodrigo
Boertjes, Emma
Bozhilova, Stela
Pijnappel, W.W.M. Pim
Schaaf, Gerben J.
author_sort Canibano-Fraile, Rodrigo
collection PubMed
description Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.
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spelling pubmed-80950532021-05-13 An in vitro assay to quantify satellite cell activation using isolated mouse myofibers Canibano-Fraile, Rodrigo Boertjes, Emma Bozhilova, Stela Pijnappel, W.W.M. Pim Schaaf, Gerben J. STAR Protoc Protocol Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation. Elsevier 2021-04-23 /pmc/articles/PMC8095053/ /pubmed/33997810 http://dx.doi.org/10.1016/j.xpro.2021.100482 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Canibano-Fraile, Rodrigo
Boertjes, Emma
Bozhilova, Stela
Pijnappel, W.W.M. Pim
Schaaf, Gerben J.
An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title_full An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title_fullStr An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title_full_unstemmed An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title_short An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
title_sort in vitro assay to quantify satellite cell activation using isolated mouse myofibers
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095053/
https://www.ncbi.nlm.nih.gov/pubmed/33997810
http://dx.doi.org/10.1016/j.xpro.2021.100482
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