Comparative evaluation of qualitative and quantitative remineralization potential of four different remineralizing agents in enamel using energy-dispersive X-ray: An in vitro study

BACKGROUND: The principles of minimally invasive dentistry clearly tells us the need for clinically effective measures to remineralize the early enamel caries lesions. AIMS AND OBJECTIVES: The aim of this study was to evaluate the remineralisation potential of four different remineralisation agents used quantitatively by surface microhardness and qualitatively by energy dispersive X ray analysis. MATERIALS AND METHODS: Artificial enamel lesions were created on the buccal surfaces of 60 extracted mandibular second premolar. Specimens were randomly assigned to four groups (n=15) according to the remineralisation agents used: Group 1-Nano Hydroxyapatite, Group 2-Fluoride, Group 3-CPP ACP, Group 4-Chitosan 5mg. All products were applied according to the manufacturer's instructions and specimens were stored in daily renewed artificial saliva. Surface microhardness was assessed using Rockwell hardness test and change in mineral content was evaluated using Energy Dispersive X ray analysis. STATISTICAL ANALYSIS: One way analysis of Variance test and post-hoc Tukey test were conducted for multiple group comparison. RESULTS: There was remarkable remineralisation in Hydroxyapatite treated comparatively to the other three groups. CONCLUSION: All remineralising agents showed improved surface remineralisation. However complete remineralisation did not occur within 7 days. Nanohydroxyapatite showed the highest potential for remineralisation followed by CPP-ACP, Chitosan and Fluoride.