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The canonical α-SNAP is essential for gametophytic development in Arabidopsis
The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096068/ https://www.ncbi.nlm.nih.gov/pubmed/33886546 http://dx.doi.org/10.1371/journal.pgen.1009505 |
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author | Liu, Fei Li, Ji-Peng Li, Lu-Shen Liu, Qi Li, Shan-Wei Song, Ming-Lei Li, Sha Zhang, Yan |
author_facet | Liu, Fei Li, Ji-Peng Li, Lu-Shen Liu, Qi Li, Shan-Wei Song, Ming-Lei Li, Sha Zhang, Yan |
author_sort | Liu, Fei |
collection | PubMed |
description | The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) complex, whose disassembly in turn ensures the recycle of individual SNARE components. The disassembly of post-fusion SNARE complexes is controlled by the AAA(+) ATPase N-ethylmaleimide-sensitive factor (Sec18/NSF) and soluble NSF attachment protein (Sec17/α-SNAP) in yeast and metazoans. Although non-canonical α-SNAPs have been functionally characterized in soybeans, the biological function of canonical α-SNAPs has yet to be demonstrated in plants. We report here that the canonical α-SNAP in Arabidopsis is essential for male and female gametophytic development. Functional loss of the canonical α-SNAP in Arabidopsis results in gametophytic lethality by arresting the first mitosis during gametogenesis. We further show that Arabidopsis α-SNAP encodes two isoforms due to alternative splicing. Both isoforms interact with the Arabidopsis homolog of NSF whereas have distinct subcellular localizations. The presence of similar alternative splicing of human α-SNAP indicates that functional distinction of two α-SNAP isoforms is evolutionarily conserved. |
format | Online Article Text |
id | pubmed-8096068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80960682021-05-13 The canonical α-SNAP is essential for gametophytic development in Arabidopsis Liu, Fei Li, Ji-Peng Li, Lu-Shen Liu, Qi Li, Shan-Wei Song, Ming-Lei Li, Sha Zhang, Yan PLoS Genet Research Article The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) complex, whose disassembly in turn ensures the recycle of individual SNARE components. The disassembly of post-fusion SNARE complexes is controlled by the AAA(+) ATPase N-ethylmaleimide-sensitive factor (Sec18/NSF) and soluble NSF attachment protein (Sec17/α-SNAP) in yeast and metazoans. Although non-canonical α-SNAPs have been functionally characterized in soybeans, the biological function of canonical α-SNAPs has yet to be demonstrated in plants. We report here that the canonical α-SNAP in Arabidopsis is essential for male and female gametophytic development. Functional loss of the canonical α-SNAP in Arabidopsis results in gametophytic lethality by arresting the first mitosis during gametogenesis. We further show that Arabidopsis α-SNAP encodes two isoforms due to alternative splicing. Both isoforms interact with the Arabidopsis homolog of NSF whereas have distinct subcellular localizations. The presence of similar alternative splicing of human α-SNAP indicates that functional distinction of two α-SNAP isoforms is evolutionarily conserved. Public Library of Science 2021-04-22 /pmc/articles/PMC8096068/ /pubmed/33886546 http://dx.doi.org/10.1371/journal.pgen.1009505 Text en © 2021 Liu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Liu, Fei Li, Ji-Peng Li, Lu-Shen Liu, Qi Li, Shan-Wei Song, Ming-Lei Li, Sha Zhang, Yan The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title | The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title_full | The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title_fullStr | The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title_full_unstemmed | The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title_short | The canonical α-SNAP is essential for gametophytic development in Arabidopsis |
title_sort | canonical α-snap is essential for gametophytic development in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096068/ https://www.ncbi.nlm.nih.gov/pubmed/33886546 http://dx.doi.org/10.1371/journal.pgen.1009505 |
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