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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification

Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions s...

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Autores principales: Zhai, Jingjing, Wang, Limin, Qiao, Xiaoliang, Zhao, Jianping, Wang, Xuexia, He, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096098/
https://www.ncbi.nlm.nih.gov/pubmed/33945580
http://dx.doi.org/10.1371/journal.pone.0251119
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author Zhai, Jingjing
Wang, Limin
Qiao, Xiaoliang
Zhao, Jianping
Wang, Xuexia
He, Xiaohong
author_facet Zhai, Jingjing
Wang, Limin
Qiao, Xiaoliang
Zhao, Jianping
Wang, Xuexia
He, Xiaohong
author_sort Zhai, Jingjing
collection PubMed
description Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66–97.29) for NG detection and 90.90 (95% CI at 57.12–99.52) for CT detection.
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spelling pubmed-80960982021-05-17 Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification Zhai, Jingjing Wang, Limin Qiao, Xiaoliang Zhao, Jianping Wang, Xuexia He, Xiaohong PLoS One Research Article Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66–97.29) for NG detection and 90.90 (95% CI at 57.12–99.52) for CT detection. Public Library of Science 2021-05-04 /pmc/articles/PMC8096098/ /pubmed/33945580 http://dx.doi.org/10.1371/journal.pone.0251119 Text en © 2021 Zhai et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhai, Jingjing
Wang, Limin
Qiao, Xiaoliang
Zhao, Jianping
Wang, Xuexia
He, Xiaohong
Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title_full Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title_fullStr Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title_full_unstemmed Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title_short Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
title_sort detection of neisseria gonorrhoeae and chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096098/
https://www.ncbi.nlm.nih.gov/pubmed/33945580
http://dx.doi.org/10.1371/journal.pone.0251119
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