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Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America
Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096431/ https://www.ncbi.nlm.nih.gov/pubmed/33942718 http://dx.doi.org/10.7554/eLife.65091 |
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author | Pessotti, Rita de Cassia Hansen, Bridget L Reaso, Jewel N Ceja-Navarro, Javier A El-Hifnawi, Laila Brodie, Eoin L Traxler, Matthew F |
author_facet | Pessotti, Rita de Cassia Hansen, Bridget L Reaso, Jewel N Ceja-Navarro, Javier A El-Hifnawi, Laila Brodie, Eoin L Traxler, Matthew F |
author_sort | Pessotti, Rita de Cassia |
collection | PubMed |
description | Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of the species Odontotaenius disjunctus, and their frass (fecal material), which is an important food resource within their galleries, as a model insect/actinomycete system. Through chemical and phylogenetic analyses, we found that O. disjunctus frass collected across eastern North America harbored multiple lineages of Streptomyces and diverse antimicrobials. Metabolites detected in frass displayed synergistic and antagonistic inhibition of a fungal entomopathogen, Metarhizium anisopliae, and multiple streptomycete isolates inhibited this pathogen when co-cultivated directly in frass. These findings support a model in which the lifestyle of O. disjunctus accommodates multiple Streptomyces lineages in their frass, resulting in a rich repertoire of antimicrobials that likely insulates their galleries against pathogenic invasion. |
format | Online Article Text |
id | pubmed-8096431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80964312021-05-06 Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America Pessotti, Rita de Cassia Hansen, Bridget L Reaso, Jewel N Ceja-Navarro, Javier A El-Hifnawi, Laila Brodie, Eoin L Traxler, Matthew F eLife Ecology Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of the species Odontotaenius disjunctus, and their frass (fecal material), which is an important food resource within their galleries, as a model insect/actinomycete system. Through chemical and phylogenetic analyses, we found that O. disjunctus frass collected across eastern North America harbored multiple lineages of Streptomyces and diverse antimicrobials. Metabolites detected in frass displayed synergistic and antagonistic inhibition of a fungal entomopathogen, Metarhizium anisopliae, and multiple streptomycete isolates inhibited this pathogen when co-cultivated directly in frass. These findings support a model in which the lifestyle of O. disjunctus accommodates multiple Streptomyces lineages in their frass, resulting in a rich repertoire of antimicrobials that likely insulates their galleries against pathogenic invasion. eLife Sciences Publications, Ltd 2021-05-04 /pmc/articles/PMC8096431/ /pubmed/33942718 http://dx.doi.org/10.7554/eLife.65091 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Ecology Pessotti, Rita de Cassia Hansen, Bridget L Reaso, Jewel N Ceja-Navarro, Javier A El-Hifnawi, Laila Brodie, Eoin L Traxler, Matthew F Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title | Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title_full | Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title_fullStr | Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title_full_unstemmed | Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title_short | Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America |
title_sort | multiple lineages of streptomyces produce antimicrobials within passalid beetle galleries across eastern north america |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096431/ https://www.ncbi.nlm.nih.gov/pubmed/33942718 http://dx.doi.org/10.7554/eLife.65091 |
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