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Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach

Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic r...

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Autores principales: Kim, Sangnam, Moon, Sunho, Rho, Sunghoon, Yoon, Sangpil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096457/
https://www.ncbi.nlm.nih.gov/pubmed/33981116
http://dx.doi.org/10.1063/5.0044512
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author Kim, Sangnam
Moon, Sunho
Rho, Sunghoon
Yoon, Sangpil
author_facet Kim, Sangnam
Moon, Sunho
Rho, Sunghoon
Yoon, Sangpil
author_sort Kim, Sangnam
collection PubMed
description Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μN. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer.
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spelling pubmed-80964572021-05-11 Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach Kim, Sangnam Moon, Sunho Rho, Sunghoon Yoon, Sangpil Appl Phys Lett Interdisciplinary Applied Physics Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μN. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer. AIP Publishing LLC 2021-05-03 2021-05-03 /pmc/articles/PMC8096457/ /pubmed/33981116 http://dx.doi.org/10.1063/5.0044512 Text en © 2021 Author(s). https://creativecommons.org/licenses/by/4.0/© 2021 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Interdisciplinary Applied Physics
Kim, Sangnam
Moon, Sunho
Rho, Sunghoon
Yoon, Sangpil
Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title_full Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title_fullStr Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title_full_unstemmed Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title_short Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
title_sort measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
topic Interdisciplinary Applied Physics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096457/
https://www.ncbi.nlm.nih.gov/pubmed/33981116
http://dx.doi.org/10.1063/5.0044512
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