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Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach
Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic r...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096457/ https://www.ncbi.nlm.nih.gov/pubmed/33981116 http://dx.doi.org/10.1063/5.0044512 |
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author | Kim, Sangnam Moon, Sunho Rho, Sunghoon Yoon, Sangpil |
author_facet | Kim, Sangnam Moon, Sunho Rho, Sunghoon Yoon, Sangpil |
author_sort | Kim, Sangnam |
collection | PubMed |
description | Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μN. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer. |
format | Online Article Text |
id | pubmed-8096457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-80964572021-05-11 Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach Kim, Sangnam Moon, Sunho Rho, Sunghoon Yoon, Sangpil Appl Phys Lett Interdisciplinary Applied Physics Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μN. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer. AIP Publishing LLC 2021-05-03 2021-05-03 /pmc/articles/PMC8096457/ /pubmed/33981116 http://dx.doi.org/10.1063/5.0044512 Text en © 2021 Author(s). https://creativecommons.org/licenses/by/4.0/© 2021 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Interdisciplinary Applied Physics Kim, Sangnam Moon, Sunho Rho, Sunghoon Yoon, Sangpil Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title_full | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title_fullStr | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title_full_unstemmed | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title_short | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
title_sort | measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach |
topic | Interdisciplinary Applied Physics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096457/ https://www.ncbi.nlm.nih.gov/pubmed/33981116 http://dx.doi.org/10.1063/5.0044512 |
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