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Acute stress reveals different impacts in male and female Zdhhc7-deficient mice
Numerous processes of neuronal development and synaptic plasticity in the brain rely on the palmitoyl acyltransferase ZDHHC7, as it palmitoylates various synaptic and extrasynaptic proteins such as neural cell adhesion molecule (NCAM) or gamma-aminobutyric acid (GABA(A)) receptors. In addition, ZDHH...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096773/ https://www.ncbi.nlm.nih.gov/pubmed/33880616 http://dx.doi.org/10.1007/s00429-021-02275-y |
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author | Kerkenberg, Nicole Hohoff, Christa Zhang, Mingyue Lang, Ilona Schettler, Christiane Ponimaskin, Evgeni Wachsmuth, Lydia Faber, Cornelius Baune, Bernhard T. Zhang, Weiqi |
author_facet | Kerkenberg, Nicole Hohoff, Christa Zhang, Mingyue Lang, Ilona Schettler, Christiane Ponimaskin, Evgeni Wachsmuth, Lydia Faber, Cornelius Baune, Bernhard T. Zhang, Weiqi |
author_sort | Kerkenberg, Nicole |
collection | PubMed |
description | Numerous processes of neuronal development and synaptic plasticity in the brain rely on the palmitoyl acyltransferase ZDHHC7, as it palmitoylates various synaptic and extrasynaptic proteins such as neural cell adhesion molecule (NCAM) or gamma-aminobutyric acid (GABA(A)) receptors. In addition, ZDHHC7 palmitoylates sex steroid hormone receptors and is, therefore, indirectly linked to mental disorders that often occur because of or in conjunction with stress. In this work, we investigated how ZDHHC7 affects stress responses in mice. For this purpose, genetically modified mice with a knockout of the Zdhhc7 gene (KO) and wild-type (WT) littermates of both sexes were exposed to acute stressors or control conditions and examined with regard to their behavior, brain microstructure, gene expression, and synaptic plasticity. While no behavioral effects of acute stress were found, we did find that acute stress caused reduced mRNA levels of Esr1 and Esr2 coding for estrogen receptor α and β in the medial prefrontal cortex of male WT and KO mice. Strikingly, after acute stress only male KO mice showed reduced mean fiber lengths of the medioventral hippocampus. Furthermore, Zdhhc7-deficiency impaired synaptic plasticity in mice of both sexes, while acute stress improved it in females, but not in male mice. Taken together, our findings suggest that ZDHHC7 plays a modulatory role in the brain that leads to sex-specific stress responses, possibly due to estrogen receptor-mediated signaling pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00429-021-02275-y. |
format | Online Article Text |
id | pubmed-8096773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-80967732021-05-05 Acute stress reveals different impacts in male and female Zdhhc7-deficient mice Kerkenberg, Nicole Hohoff, Christa Zhang, Mingyue Lang, Ilona Schettler, Christiane Ponimaskin, Evgeni Wachsmuth, Lydia Faber, Cornelius Baune, Bernhard T. Zhang, Weiqi Brain Struct Funct Original Article Numerous processes of neuronal development and synaptic plasticity in the brain rely on the palmitoyl acyltransferase ZDHHC7, as it palmitoylates various synaptic and extrasynaptic proteins such as neural cell adhesion molecule (NCAM) or gamma-aminobutyric acid (GABA(A)) receptors. In addition, ZDHHC7 palmitoylates sex steroid hormone receptors and is, therefore, indirectly linked to mental disorders that often occur because of or in conjunction with stress. In this work, we investigated how ZDHHC7 affects stress responses in mice. For this purpose, genetically modified mice with a knockout of the Zdhhc7 gene (KO) and wild-type (WT) littermates of both sexes were exposed to acute stressors or control conditions and examined with regard to their behavior, brain microstructure, gene expression, and synaptic plasticity. While no behavioral effects of acute stress were found, we did find that acute stress caused reduced mRNA levels of Esr1 and Esr2 coding for estrogen receptor α and β in the medial prefrontal cortex of male WT and KO mice. Strikingly, after acute stress only male KO mice showed reduced mean fiber lengths of the medioventral hippocampus. Furthermore, Zdhhc7-deficiency impaired synaptic plasticity in mice of both sexes, while acute stress improved it in females, but not in male mice. Taken together, our findings suggest that ZDHHC7 plays a modulatory role in the brain that leads to sex-specific stress responses, possibly due to estrogen receptor-mediated signaling pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00429-021-02275-y. Springer Berlin Heidelberg 2021-04-20 2021 /pmc/articles/PMC8096773/ /pubmed/33880616 http://dx.doi.org/10.1007/s00429-021-02275-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Kerkenberg, Nicole Hohoff, Christa Zhang, Mingyue Lang, Ilona Schettler, Christiane Ponimaskin, Evgeni Wachsmuth, Lydia Faber, Cornelius Baune, Bernhard T. Zhang, Weiqi Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title | Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title_full | Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title_fullStr | Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title_full_unstemmed | Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title_short | Acute stress reveals different impacts in male and female Zdhhc7-deficient mice |
title_sort | acute stress reveals different impacts in male and female zdhhc7-deficient mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096773/ https://www.ncbi.nlm.nih.gov/pubmed/33880616 http://dx.doi.org/10.1007/s00429-021-02275-y |
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