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Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet

The present work is aimed to examine the genetic variability and the distribution pattern of beneficial Trichoderma spp. isolated from rhizosphere samples and their mode of action in improving the plant health. A total of 131 suspected fungi were isolated from the rhizospheric soil and 91 isolates w...

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Autores principales: Nandini, Boregowda, Puttaswamy, Hariprasad, Saini, Ramesh Kumar, Prakash, Harischandra Sripathy, Geetha, Nagaraja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096818/
https://www.ncbi.nlm.nih.gov/pubmed/33947949
http://dx.doi.org/10.1038/s41598-021-89061-2
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author Nandini, Boregowda
Puttaswamy, Hariprasad
Saini, Ramesh Kumar
Prakash, Harischandra Sripathy
Geetha, Nagaraja
author_facet Nandini, Boregowda
Puttaswamy, Hariprasad
Saini, Ramesh Kumar
Prakash, Harischandra Sripathy
Geetha, Nagaraja
author_sort Nandini, Boregowda
collection PubMed
description The present work is aimed to examine the genetic variability and the distribution pattern of beneficial Trichoderma spp. isolated from rhizosphere samples and their mode of action in improving the plant health. A total of 131 suspected fungi were isolated from the rhizospheric soil and 91 isolates were confirmed as Trichoderma spp. T. asperellum and T. harzianum were found high in the frequency of occurrence. Genetic diversity analysis using RAPD and ISSR revealed the diverse distribution pattern of Trichoderma spp. indicating their capability to adapt to broad agroclimatic conditions. Analysis of genetic diversity using molecular markers revealed intra-species diversity of isolated Trichoderma spp. The frequency of pearl millet (PM) root colonization by Trichoderma spp. was found to be 100%. However, they showed varied results for indole acetic acid, siderophore, phosphate solubilization, β-1,3-glucanase, chitinase, cellulase, lipase, and protease activity. Downy mildew disease protection studies revealed a strong involvement of Trichoderma spp. in direct suppression of the pathogen (mean 37.41) in the rhizosphere followed by inducing systemic resistance. Our findings highlights the probable distribution and diversity profile of Trichoderma spp. as well as narrate the possible utilization of Trichoderma spp. as microbial fungicides in PM cultivation across different agroclimatic zones of India.
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spelling pubmed-80968182021-05-05 Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet Nandini, Boregowda Puttaswamy, Hariprasad Saini, Ramesh Kumar Prakash, Harischandra Sripathy Geetha, Nagaraja Sci Rep Article The present work is aimed to examine the genetic variability and the distribution pattern of beneficial Trichoderma spp. isolated from rhizosphere samples and their mode of action in improving the plant health. A total of 131 suspected fungi were isolated from the rhizospheric soil and 91 isolates were confirmed as Trichoderma spp. T. asperellum and T. harzianum were found high in the frequency of occurrence. Genetic diversity analysis using RAPD and ISSR revealed the diverse distribution pattern of Trichoderma spp. indicating their capability to adapt to broad agroclimatic conditions. Analysis of genetic diversity using molecular markers revealed intra-species diversity of isolated Trichoderma spp. The frequency of pearl millet (PM) root colonization by Trichoderma spp. was found to be 100%. However, they showed varied results for indole acetic acid, siderophore, phosphate solubilization, β-1,3-glucanase, chitinase, cellulase, lipase, and protease activity. Downy mildew disease protection studies revealed a strong involvement of Trichoderma spp. in direct suppression of the pathogen (mean 37.41) in the rhizosphere followed by inducing systemic resistance. Our findings highlights the probable distribution and diversity profile of Trichoderma spp. as well as narrate the possible utilization of Trichoderma spp. as microbial fungicides in PM cultivation across different agroclimatic zones of India. Nature Publishing Group UK 2021-05-04 /pmc/articles/PMC8096818/ /pubmed/33947949 http://dx.doi.org/10.1038/s41598-021-89061-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nandini, Boregowda
Puttaswamy, Hariprasad
Saini, Ramesh Kumar
Prakash, Harischandra Sripathy
Geetha, Nagaraja
Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title_full Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title_fullStr Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title_full_unstemmed Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title_short Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
title_sort trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096818/
https://www.ncbi.nlm.nih.gov/pubmed/33947949
http://dx.doi.org/10.1038/s41598-021-89061-2
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