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Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats

Exercising was reported by several studies to bring great benefits to heart failure with preserved ejection fraction (HFpEF), which reduced the hospitalization and the mortality of heart failure. However, the underlying mechanism of exercising on HFpEF remains unclear. In the present study, we desig...

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Autores principales: Liu, Jian, Ji, Xi-xin, Fu, Yang, Zhang, Wen-chao, Ji, Hui-fang, Liu, Jian-wei, Cheng, Xiao-shu, Dong, Yi-Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096912/
https://www.ncbi.nlm.nih.gov/pubmed/33969019
http://dx.doi.org/10.3389/fcvm.2021.655009
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author Liu, Jian
Ji, Xi-xin
Fu, Yang
Zhang, Wen-chao
Ji, Hui-fang
Liu, Jian-wei
Cheng, Xiao-shu
Dong, Yi-Fei
author_facet Liu, Jian
Ji, Xi-xin
Fu, Yang
Zhang, Wen-chao
Ji, Hui-fang
Liu, Jian-wei
Cheng, Xiao-shu
Dong, Yi-Fei
author_sort Liu, Jian
collection PubMed
description Exercising was reported by several studies to bring great benefits to heart failure with preserved ejection fraction (HFpEF), which reduced the hospitalization and the mortality of heart failure. However, the underlying mechanism of exercising on HFpEF remains unclear. In the present study, we designed and constructed a device that can perform early passive leg movement (ePLM) in rats and further observed whether treatment of ePLM exerts protective effects on HFpEF of rats. Rats were fed with high salt feed to establish an animal model of pre-clinical diastolic dysfunction (PDD), which would eventually develop into HFpEF, and then treated rats with ePLM. We conducted several experiments to evaluate the conditions of heart and blood vessel. The results show that diastolic functions of heart and blood vessel in rats were significantly improved by treatment of ePLM. We also found that pathological injuries of heart and blood vessel were ameliorated after treatment of ePLM. Moreover, treatment of ePLM decreased the protein levels of Collagen type I, Collagen type III, MMP2, and MMP9 in heart and blood vessel, indicating that cardiac and vascular fibrosis were reduced apparently by treatment of ePLM. Further investigation suggested that treatment of ePLM probably inhibit the activation of TGF-β1/Smad3 signaling pathway as well as promote the activation of Akt/eNOS signaling pathway in high salt diet induced HFpEF. In conclusion, treatment of ePLM alleviated high salt diet induced HFpEF by inhibiting fibrosis via suppressing TGF-β1/Smad3 signaling pathway as well as activating Akt/eNOS signaling pathway, implicating treatment of ePLM as a promising novel non-pharmacological approach for HFpEF.
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spelling pubmed-80969122021-05-06 Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats Liu, Jian Ji, Xi-xin Fu, Yang Zhang, Wen-chao Ji, Hui-fang Liu, Jian-wei Cheng, Xiao-shu Dong, Yi-Fei Front Cardiovasc Med Cardiovascular Medicine Exercising was reported by several studies to bring great benefits to heart failure with preserved ejection fraction (HFpEF), which reduced the hospitalization and the mortality of heart failure. However, the underlying mechanism of exercising on HFpEF remains unclear. In the present study, we designed and constructed a device that can perform early passive leg movement (ePLM) in rats and further observed whether treatment of ePLM exerts protective effects on HFpEF of rats. Rats were fed with high salt feed to establish an animal model of pre-clinical diastolic dysfunction (PDD), which would eventually develop into HFpEF, and then treated rats with ePLM. We conducted several experiments to evaluate the conditions of heart and blood vessel. The results show that diastolic functions of heart and blood vessel in rats were significantly improved by treatment of ePLM. We also found that pathological injuries of heart and blood vessel were ameliorated after treatment of ePLM. Moreover, treatment of ePLM decreased the protein levels of Collagen type I, Collagen type III, MMP2, and MMP9 in heart and blood vessel, indicating that cardiac and vascular fibrosis were reduced apparently by treatment of ePLM. Further investigation suggested that treatment of ePLM probably inhibit the activation of TGF-β1/Smad3 signaling pathway as well as promote the activation of Akt/eNOS signaling pathway in high salt diet induced HFpEF. In conclusion, treatment of ePLM alleviated high salt diet induced HFpEF by inhibiting fibrosis via suppressing TGF-β1/Smad3 signaling pathway as well as activating Akt/eNOS signaling pathway, implicating treatment of ePLM as a promising novel non-pharmacological approach for HFpEF. Frontiers Media S.A. 2021-04-21 /pmc/articles/PMC8096912/ /pubmed/33969019 http://dx.doi.org/10.3389/fcvm.2021.655009 Text en Copyright © 2021 Liu, Ji, Fu, Zhang, Ji, Liu, Cheng and Dong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Liu, Jian
Ji, Xi-xin
Fu, Yang
Zhang, Wen-chao
Ji, Hui-fang
Liu, Jian-wei
Cheng, Xiao-shu
Dong, Yi-Fei
Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title_full Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title_fullStr Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title_full_unstemmed Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title_short Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats
title_sort early passive leg movement prevents against the development of heart failure with preserved ejection fraction in rats
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096912/
https://www.ncbi.nlm.nih.gov/pubmed/33969019
http://dx.doi.org/10.3389/fcvm.2021.655009
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