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Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes

Exploring the natural genetic variability and its exploitation for improved Nitrogen Use Efficiency (NUE) in sorghum is one of the primary goals in the modern crop improvement programs. The integrated strategies include high-throughput phenotyping, next generation sequencing (NGS)-based genotyping t...

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Autores principales: Bollam, Srikanth, Romana, Kirandeep Kaur, Rayaprolu, Laavanya, Vemula, Anilkumar, Das, Roma Rani, Rathore, Abhishek, Gandham, Prasad, Chander, Girish, Deshpande, Santosh P., Gupta, Rajeev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097177/
https://www.ncbi.nlm.nih.gov/pubmed/33968102
http://dx.doi.org/10.3389/fpls.2021.643192
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author Bollam, Srikanth
Romana, Kirandeep Kaur
Rayaprolu, Laavanya
Vemula, Anilkumar
Das, Roma Rani
Rathore, Abhishek
Gandham, Prasad
Chander, Girish
Deshpande, Santosh P.
Gupta, Rajeev
author_facet Bollam, Srikanth
Romana, Kirandeep Kaur
Rayaprolu, Laavanya
Vemula, Anilkumar
Das, Roma Rani
Rathore, Abhishek
Gandham, Prasad
Chander, Girish
Deshpande, Santosh P.
Gupta, Rajeev
author_sort Bollam, Srikanth
collection PubMed
description Exploring the natural genetic variability and its exploitation for improved Nitrogen Use Efficiency (NUE) in sorghum is one of the primary goals in the modern crop improvement programs. The integrated strategies include high-throughput phenotyping, next generation sequencing (NGS)-based genotyping technologies, and a priori selected candidate gene studies that help understand the detailed physiological and molecular mechanisms underpinning this complex trait. A set of sixty diverse sorghum genotypes was evaluated for different vegetative, reproductive, and yield traits related to NUE in the field (under three N regimes) for two seasons. Significant variations for different yield and related traits under 0 and 50% N confirmed the availability of native genetic variability in sorghum under low N regimes. Sorghum genotypes with distinct genetic background had interestingly similar NUE associated traits. The Genotyping-By-Sequencing based SNPs (>89 K) were used to study the population structure, and phylogenetic groupings identified three distinct groups. The information of grain N and stalk N content of the individuals covered on the phylogenetic groups indicated randomness in the distribution for adaptation under variable N regimes. This study identified promising sorghum genotypes with consistent performance under varying environments, with buffer capacity for yield under low N conditions. We also report better performing genotypes for varied production use—grain, stover, and dual-purpose sorghum having differential adaptation response to NUE traits. Expression profiling of NUE associated genes in shoot and root tissues of contrasting lines (PVK801 and HDW703) grown in varying N conditions revealed interesting outcomes. Root tissues of contrasting lines exhibited differential expression profiles for transporter genes [ammonium transporter (SbAMT), nitrate transporters (SbNRT)]; primary assimilatory (glutamine synthetase (SbGS), glutamate synthase (SbGOGAT[NADH], SbGOGAT[Fd]), assimilatory genes [nitrite reductase (SbNiR[NADH]3)]; and amino acid biosynthesis associated gene [glutamate dehydrogenase (SbGDH)]. Identification and expression profiling of contrasting sorghum genotypes in varying N dosages will provide new information to understand the response of NUE genes toward adaptation to the differential N regimes in sorghum. High NUE genotypes identified from this study could be potential candidates for in-depth molecular analysis and contribute toward the development of N efficient sorghum cultivars.
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spelling pubmed-80971772021-05-06 Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes Bollam, Srikanth Romana, Kirandeep Kaur Rayaprolu, Laavanya Vemula, Anilkumar Das, Roma Rani Rathore, Abhishek Gandham, Prasad Chander, Girish Deshpande, Santosh P. Gupta, Rajeev Front Plant Sci Plant Science Exploring the natural genetic variability and its exploitation for improved Nitrogen Use Efficiency (NUE) in sorghum is one of the primary goals in the modern crop improvement programs. The integrated strategies include high-throughput phenotyping, next generation sequencing (NGS)-based genotyping technologies, and a priori selected candidate gene studies that help understand the detailed physiological and molecular mechanisms underpinning this complex trait. A set of sixty diverse sorghum genotypes was evaluated for different vegetative, reproductive, and yield traits related to NUE in the field (under three N regimes) for two seasons. Significant variations for different yield and related traits under 0 and 50% N confirmed the availability of native genetic variability in sorghum under low N regimes. Sorghum genotypes with distinct genetic background had interestingly similar NUE associated traits. The Genotyping-By-Sequencing based SNPs (>89 K) were used to study the population structure, and phylogenetic groupings identified three distinct groups. The information of grain N and stalk N content of the individuals covered on the phylogenetic groups indicated randomness in the distribution for adaptation under variable N regimes. This study identified promising sorghum genotypes with consistent performance under varying environments, with buffer capacity for yield under low N conditions. We also report better performing genotypes for varied production use—grain, stover, and dual-purpose sorghum having differential adaptation response to NUE traits. Expression profiling of NUE associated genes in shoot and root tissues of contrasting lines (PVK801 and HDW703) grown in varying N conditions revealed interesting outcomes. Root tissues of contrasting lines exhibited differential expression profiles for transporter genes [ammonium transporter (SbAMT), nitrate transporters (SbNRT)]; primary assimilatory (glutamine synthetase (SbGS), glutamate synthase (SbGOGAT[NADH], SbGOGAT[Fd]), assimilatory genes [nitrite reductase (SbNiR[NADH]3)]; and amino acid biosynthesis associated gene [glutamate dehydrogenase (SbGDH)]. Identification and expression profiling of contrasting sorghum genotypes in varying N dosages will provide new information to understand the response of NUE genes toward adaptation to the differential N regimes in sorghum. High NUE genotypes identified from this study could be potential candidates for in-depth molecular analysis and contribute toward the development of N efficient sorghum cultivars. Frontiers Media S.A. 2021-04-21 /pmc/articles/PMC8097177/ /pubmed/33968102 http://dx.doi.org/10.3389/fpls.2021.643192 Text en Copyright © 2021 Bollam, Romana, Rayaprolu, Vemula, Das, Rathore, Gandham, Chander, Deshpande and Gupta. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bollam, Srikanth
Romana, Kirandeep Kaur
Rayaprolu, Laavanya
Vemula, Anilkumar
Das, Roma Rani
Rathore, Abhishek
Gandham, Prasad
Chander, Girish
Deshpande, Santosh P.
Gupta, Rajeev
Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title_full Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title_fullStr Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title_full_unstemmed Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title_short Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
title_sort nitrogen use efficiency in sorghum: exploring native variability for traits under variable n-regimes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097177/
https://www.ncbi.nlm.nih.gov/pubmed/33968102
http://dx.doi.org/10.3389/fpls.2021.643192
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