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Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway
Nicotinamide adenine dinucleotide (NAD(+)) is a life essential molecule involved in versatile biological processes. To date, only two de novo biosynthetic routes to NAD(+) are described, both of which start from a proteinogenic amino acid and are tightly controlled. Here, a de novo quinolinic acid p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097395/ https://www.ncbi.nlm.nih.gov/pubmed/33977072 http://dx.doi.org/10.1002/advs.202004632 |
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author | Ding, Yong Li, Xinli Horsman, Geoff P. Li, Pengwei Wang, Min Li, Jine Zhang, Zhilong Liu, Weifeng Wu, Bian Tao, Yong Chen, Yihua |
author_facet | Ding, Yong Li, Xinli Horsman, Geoff P. Li, Pengwei Wang, Min Li, Jine Zhang, Zhilong Liu, Weifeng Wu, Bian Tao, Yong Chen, Yihua |
author_sort | Ding, Yong |
collection | PubMed |
description | Nicotinamide adenine dinucleotide (NAD(+)) is a life essential molecule involved in versatile biological processes. To date, only two de novo biosynthetic routes to NAD(+) are described, both of which start from a proteinogenic amino acid and are tightly controlled. Here, a de novo quinolinic acid pathway starting from chorismate, which provides an alternative route (named as the C3N pathway) to NAD(+) biosynthesis, is established. Significantly, the C3N pathway yields extremely high cellular concentrations of NAD(H) in E. coli. Its utility in cofactor engineering is demonstrated by introducing the four‐gene C3N module to cell factories to achieve higher production of 2,5‐dimethylpyrazine and develop an efficient C3N‐based whole‐cell bioconversion system for preparing chiral amines. The wide distribution and abundance of chorismate in most kingdoms of life implies a general utility of the C3N pathway for modulating cellular levels of NAD(H) in versatile organisms. |
format | Online Article Text |
id | pubmed-8097395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80973952021-05-10 Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway Ding, Yong Li, Xinli Horsman, Geoff P. Li, Pengwei Wang, Min Li, Jine Zhang, Zhilong Liu, Weifeng Wu, Bian Tao, Yong Chen, Yihua Adv Sci (Weinh) Research Articles Nicotinamide adenine dinucleotide (NAD(+)) is a life essential molecule involved in versatile biological processes. To date, only two de novo biosynthetic routes to NAD(+) are described, both of which start from a proteinogenic amino acid and are tightly controlled. Here, a de novo quinolinic acid pathway starting from chorismate, which provides an alternative route (named as the C3N pathway) to NAD(+) biosynthesis, is established. Significantly, the C3N pathway yields extremely high cellular concentrations of NAD(H) in E. coli. Its utility in cofactor engineering is demonstrated by introducing the four‐gene C3N module to cell factories to achieve higher production of 2,5‐dimethylpyrazine and develop an efficient C3N‐based whole‐cell bioconversion system for preparing chiral amines. The wide distribution and abundance of chorismate in most kingdoms of life implies a general utility of the C3N pathway for modulating cellular levels of NAD(H) in versatile organisms. John Wiley and Sons Inc. 2021-03-01 /pmc/articles/PMC8097395/ /pubmed/33977072 http://dx.doi.org/10.1002/advs.202004632 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ding, Yong Li, Xinli Horsman, Geoff P. Li, Pengwei Wang, Min Li, Jine Zhang, Zhilong Liu, Weifeng Wu, Bian Tao, Yong Chen, Yihua Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title | Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title_full | Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title_fullStr | Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title_full_unstemmed | Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title_short | Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway |
title_sort | construction of an alternative nad(+) de novo biosynthesis pathway |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097395/ https://www.ncbi.nlm.nih.gov/pubmed/33977072 http://dx.doi.org/10.1002/advs.202004632 |
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