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Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097490/ https://www.ncbi.nlm.nih.gov/pubmed/33939796 http://dx.doi.org/10.1083/jcb.202007055 |
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author | Herrera, Antonio Menendez, Anghara Torroba, Blanca Ochoa, Andrea Pons, Sebastián |
author_facet | Herrera, Antonio Menendez, Anghara Torroba, Blanca Ochoa, Andrea Pons, Sebastián |
author_sort | Herrera, Antonio |
collection | PubMed |
description | The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formation of enlarged apical complexes (ACs), enhancing apico-basal polarity, although the mechanism through which this occurs remained unclear. Here, we show that β-catenin interacts with phosphorylated pro-N-cadherin early in its transit through the Golgi apparatus, promoting propeptide excision and the final maturation of N-cadherin. We describe a new β-catenin–dependent interaction of N-cadherin with Drebrin-like (Dbnl), an actin-binding protein that is involved in anterograde Golgi trafficking of proteins. Notably, Dbnl knockdown led to pro-N-cadherin accumulation and limited AJ formation. In brief, we demonstrate that Dbnl and β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity. |
format | Online Article Text |
id | pubmed-8097490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80974902021-12-07 Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity Herrera, Antonio Menendez, Anghara Torroba, Blanca Ochoa, Andrea Pons, Sebastián J Cell Biol Article The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formation of enlarged apical complexes (ACs), enhancing apico-basal polarity, although the mechanism through which this occurs remained unclear. Here, we show that β-catenin interacts with phosphorylated pro-N-cadherin early in its transit through the Golgi apparatus, promoting propeptide excision and the final maturation of N-cadherin. We describe a new β-catenin–dependent interaction of N-cadherin with Drebrin-like (Dbnl), an actin-binding protein that is involved in anterograde Golgi trafficking of proteins. Notably, Dbnl knockdown led to pro-N-cadherin accumulation and limited AJ formation. In brief, we demonstrate that Dbnl and β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity. Rockefeller University Press 2021-05-03 /pmc/articles/PMC8097490/ /pubmed/33939796 http://dx.doi.org/10.1083/jcb.202007055 Text en © 2021 Herrera et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Herrera, Antonio Menendez, Anghara Torroba, Blanca Ochoa, Andrea Pons, Sebastián Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title | Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title_full | Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title_fullStr | Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title_full_unstemmed | Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title_short | Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity |
title_sort | dbnl and β-catenin promote pro-n-cadherin processing to maintain apico-basal polarity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097490/ https://www.ncbi.nlm.nih.gov/pubmed/33939796 http://dx.doi.org/10.1083/jcb.202007055 |
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