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Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity

The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formati...

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Autores principales: Herrera, Antonio, Menendez, Anghara, Torroba, Blanca, Ochoa, Andrea, Pons, Sebastián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097490/
https://www.ncbi.nlm.nih.gov/pubmed/33939796
http://dx.doi.org/10.1083/jcb.202007055
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author Herrera, Antonio
Menendez, Anghara
Torroba, Blanca
Ochoa, Andrea
Pons, Sebastián
author_facet Herrera, Antonio
Menendez, Anghara
Torroba, Blanca
Ochoa, Andrea
Pons, Sebastián
author_sort Herrera, Antonio
collection PubMed
description The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formation of enlarged apical complexes (ACs), enhancing apico-basal polarity, although the mechanism through which this occurs remained unclear. Here, we show that β-catenin interacts with phosphorylated pro-N-cadherin early in its transit through the Golgi apparatus, promoting propeptide excision and the final maturation of N-cadherin. We describe a new β-catenin–dependent interaction of N-cadherin with Drebrin-like (Dbnl), an actin-binding protein that is involved in anterograde Golgi trafficking of proteins. Notably, Dbnl knockdown led to pro-N-cadherin accumulation and limited AJ formation. In brief, we demonstrate that Dbnl and β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity.
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spelling pubmed-80974902021-12-07 Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity Herrera, Antonio Menendez, Anghara Torroba, Blanca Ochoa, Andrea Pons, Sebastián J Cell Biol Article The neural tube forms when neural stem cells arrange into a pseudostratified, single-cell–layered epithelium, with a marked apico-basal polarity, and in which adherens junctions (AJs) concentrate in the subapical domain. We previously reported that sustained β-catenin expression promotes the formation of enlarged apical complexes (ACs), enhancing apico-basal polarity, although the mechanism through which this occurs remained unclear. Here, we show that β-catenin interacts with phosphorylated pro-N-cadherin early in its transit through the Golgi apparatus, promoting propeptide excision and the final maturation of N-cadherin. We describe a new β-catenin–dependent interaction of N-cadherin with Drebrin-like (Dbnl), an actin-binding protein that is involved in anterograde Golgi trafficking of proteins. Notably, Dbnl knockdown led to pro-N-cadherin accumulation and limited AJ formation. In brief, we demonstrate that Dbnl and β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity. Rockefeller University Press 2021-05-03 /pmc/articles/PMC8097490/ /pubmed/33939796 http://dx.doi.org/10.1083/jcb.202007055 Text en © 2021 Herrera et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Herrera, Antonio
Menendez, Anghara
Torroba, Blanca
Ochoa, Andrea
Pons, Sebastián
Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title_full Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title_fullStr Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title_full_unstemmed Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title_short Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity
title_sort dbnl and β-catenin promote pro-n-cadherin processing to maintain apico-basal polarity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097490/
https://www.ncbi.nlm.nih.gov/pubmed/33939796
http://dx.doi.org/10.1083/jcb.202007055
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