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Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility
INTRODUCTION: Approximately 15% of couples in the reproductive age are struggling with infertility which, in nearly half of them, is caused by male factors. MATERIAL AND METHODS: The present study comprised of two groups of sixteen C57BL/6 mice; each mouse received either an intraperitoneal injectio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Polish Urological Association
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097657/ https://www.ncbi.nlm.nih.gov/pubmed/33976924 http://dx.doi.org/10.5173/ceju.2020.0175 |
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author | Nabighadim, Amirreza Jafarnezhad-Ansariha, Fahimeh Majidi Zolbin, Masoumeh Daryabari, Seyedeh Sima Fendereski, Kiarad Kajbafzadeh, Abdol Mohammad |
author_facet | Nabighadim, Amirreza Jafarnezhad-Ansariha, Fahimeh Majidi Zolbin, Masoumeh Daryabari, Seyedeh Sima Fendereski, Kiarad Kajbafzadeh, Abdol Mohammad |
author_sort | Nabighadim, Amirreza |
collection | PubMed |
description | INTRODUCTION: Approximately 15% of couples in the reproductive age are struggling with infertility which, in nearly half of them, is caused by male factors. MATERIAL AND METHODS: The present study comprised of two groups of sixteen C57BL/6 mice; each mouse received either an intraperitoneal injection of 30 mg/kg of an alkylating agent or the same amount of distilled water. Testes were harvested 30 days following the injection. Morphometric analysis of hematoxylin and eosin (H&E) stained slides including mean tubular area, diameter and intratubular particles were performed. Spermatogenesis rate was assessed by spermatogonial markers including promyelocytic leukemia zinc finger protein (PLZF) and neurogenin-3 (NGN3). Moreover, the expression rate of Wilms Tumor-1 (WT-1), A-Kinase Anchoring Protein 4 (AKAP4) and adenosine deaminase domain containing 1 (ADAD1) genes were evaluated via real-time polymerase chain reaction (RT-PCR). RESULTS: The body weight gradually increased in both groups after a period of 30 days, however, the increase was significantly (p-value = 0.023) lower in the chemically treated group. All the morphometric parameters were considerably decreased in the azoospermic mice. Also, promyelocytic leukemia zinc finger protein and neurogenin-3 expression dramatically declined (p-value <0.001 for both markers). In comparison with the negative control group, the expression rates of A-Kinase Anchoring Protein 4 and adenosine deaminase domain containing 1, two genes participating in the sperm structure, were remarkably reduced in the intervention group (p-value <0.001); however, our investigations demonstrated that the azoospermia model could induce a 5-fold upregulation in Wilms Tumor-1 gene expression. CONCLUSIONS: Development of an azoospermia model can upregulate Wilms Tumor-1 gene expression in a higher rate after 30 days; however, expression of the testis-specific genes, A-Kinase Anchoring Protein 4 and adenosine deaminase domain containing 1, decreased after the intervention. To the best of our knowledge, this upregulation could be related to spermatogenesis recovery after the follow-up period. |
format | Online Article Text |
id | pubmed-8097657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Polish Urological Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-80976572021-05-10 Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility Nabighadim, Amirreza Jafarnezhad-Ansariha, Fahimeh Majidi Zolbin, Masoumeh Daryabari, Seyedeh Sima Fendereski, Kiarad Kajbafzadeh, Abdol Mohammad Cent European J Urol Original Paper INTRODUCTION: Approximately 15% of couples in the reproductive age are struggling with infertility which, in nearly half of them, is caused by male factors. MATERIAL AND METHODS: The present study comprised of two groups of sixteen C57BL/6 mice; each mouse received either an intraperitoneal injection of 30 mg/kg of an alkylating agent or the same amount of distilled water. Testes were harvested 30 days following the injection. Morphometric analysis of hematoxylin and eosin (H&E) stained slides including mean tubular area, diameter and intratubular particles were performed. Spermatogenesis rate was assessed by spermatogonial markers including promyelocytic leukemia zinc finger protein (PLZF) and neurogenin-3 (NGN3). Moreover, the expression rate of Wilms Tumor-1 (WT-1), A-Kinase Anchoring Protein 4 (AKAP4) and adenosine deaminase domain containing 1 (ADAD1) genes were evaluated via real-time polymerase chain reaction (RT-PCR). RESULTS: The body weight gradually increased in both groups after a period of 30 days, however, the increase was significantly (p-value = 0.023) lower in the chemically treated group. All the morphometric parameters were considerably decreased in the azoospermic mice. Also, promyelocytic leukemia zinc finger protein and neurogenin-3 expression dramatically declined (p-value <0.001 for both markers). In comparison with the negative control group, the expression rates of A-Kinase Anchoring Protein 4 and adenosine deaminase domain containing 1, two genes participating in the sperm structure, were remarkably reduced in the intervention group (p-value <0.001); however, our investigations demonstrated that the azoospermia model could induce a 5-fold upregulation in Wilms Tumor-1 gene expression. CONCLUSIONS: Development of an azoospermia model can upregulate Wilms Tumor-1 gene expression in a higher rate after 30 days; however, expression of the testis-specific genes, A-Kinase Anchoring Protein 4 and adenosine deaminase domain containing 1, decreased after the intervention. To the best of our knowledge, this upregulation could be related to spermatogenesis recovery after the follow-up period. Polish Urological Association 2020-12-31 2021 /pmc/articles/PMC8097657/ /pubmed/33976924 http://dx.doi.org/10.5173/ceju.2020.0175 Text en Copyright by Polish Urological Association https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Original Paper Nabighadim, Amirreza Jafarnezhad-Ansariha, Fahimeh Majidi Zolbin, Masoumeh Daryabari, Seyedeh Sima Fendereski, Kiarad Kajbafzadeh, Abdol Mohammad Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title | Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title_full | Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title_fullStr | Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title_full_unstemmed | Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title_short | Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
title_sort | gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097657/ https://www.ncbi.nlm.nih.gov/pubmed/33976924 http://dx.doi.org/10.5173/ceju.2020.0175 |
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