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Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient

AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen...

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Autores principales: Feng, Yanling, Zeng, Dong, Hu, Lvyin, Yang, Yuexiang, Song, Shu, Shi, Yuhan, Xu, Jingjing, Guo, Wenjuan, Ling, Yun, Qi, Tangkai, Wu, Qingguo, Li, Feng, Cheng, Jilin, Lu, Hongzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097675/
https://www.ncbi.nlm.nih.gov/pubmed/33952310
http://dx.doi.org/10.1186/s13000-021-01082-7
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author Feng, Yanling
Zeng, Dong
Hu, Lvyin
Yang, Yuexiang
Song, Shu
Shi, Yuhan
Xu, Jingjing
Guo, Wenjuan
Ling, Yun
Qi, Tangkai
Wu, Qingguo
Li, Feng
Cheng, Jilin
Lu, Hongzhou
author_facet Feng, Yanling
Zeng, Dong
Hu, Lvyin
Yang, Yuexiang
Song, Shu
Shi, Yuhan
Xu, Jingjing
Guo, Wenjuan
Ling, Yun
Qi, Tangkai
Wu, Qingguo
Li, Feng
Cheng, Jilin
Lu, Hongzhou
author_sort Feng, Yanling
collection PubMed
description AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed. RESULTS: We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68(+) macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity. CONCLUSIONS: The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection.
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spelling pubmed-80976752021-05-05 Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient Feng, Yanling Zeng, Dong Hu, Lvyin Yang, Yuexiang Song, Shu Shi, Yuhan Xu, Jingjing Guo, Wenjuan Ling, Yun Qi, Tangkai Wu, Qingguo Li, Feng Cheng, Jilin Lu, Hongzhou Diagn Pathol Case Report AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed. RESULTS: We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68(+) macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity. CONCLUSIONS: The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection. BioMed Central 2021-05-05 /pmc/articles/PMC8097675/ /pubmed/33952310 http://dx.doi.org/10.1186/s13000-021-01082-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Case Report
Feng, Yanling
Zeng, Dong
Hu, Lvyin
Yang, Yuexiang
Song, Shu
Shi, Yuhan
Xu, Jingjing
Guo, Wenjuan
Ling, Yun
Qi, Tangkai
Wu, Qingguo
Li, Feng
Cheng, Jilin
Lu, Hongzhou
Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title_full Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title_fullStr Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title_full_unstemmed Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title_short Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
title_sort case report: histopathology and molecular pathology analysis on enteric tissue of a covid-19 patient
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097675/
https://www.ncbi.nlm.nih.gov/pubmed/33952310
http://dx.doi.org/10.1186/s13000-021-01082-7
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