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Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097675/ https://www.ncbi.nlm.nih.gov/pubmed/33952310 http://dx.doi.org/10.1186/s13000-021-01082-7 |
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author | Feng, Yanling Zeng, Dong Hu, Lvyin Yang, Yuexiang Song, Shu Shi, Yuhan Xu, Jingjing Guo, Wenjuan Ling, Yun Qi, Tangkai Wu, Qingguo Li, Feng Cheng, Jilin Lu, Hongzhou |
author_facet | Feng, Yanling Zeng, Dong Hu, Lvyin Yang, Yuexiang Song, Shu Shi, Yuhan Xu, Jingjing Guo, Wenjuan Ling, Yun Qi, Tangkai Wu, Qingguo Li, Feng Cheng, Jilin Lu, Hongzhou |
author_sort | Feng, Yanling |
collection | PubMed |
description | AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed. RESULTS: We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68(+) macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity. CONCLUSIONS: The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-8097675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80976752021-05-05 Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient Feng, Yanling Zeng, Dong Hu, Lvyin Yang, Yuexiang Song, Shu Shi, Yuhan Xu, Jingjing Guo, Wenjuan Ling, Yun Qi, Tangkai Wu, Qingguo Li, Feng Cheng, Jilin Lu, Hongzhou Diagn Pathol Case Report AIMS: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. METHODS: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed. RESULTS: We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68(+) macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity. CONCLUSIONS: The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection. BioMed Central 2021-05-05 /pmc/articles/PMC8097675/ /pubmed/33952310 http://dx.doi.org/10.1186/s13000-021-01082-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Case Report Feng, Yanling Zeng, Dong Hu, Lvyin Yang, Yuexiang Song, Shu Shi, Yuhan Xu, Jingjing Guo, Wenjuan Ling, Yun Qi, Tangkai Wu, Qingguo Li, Feng Cheng, Jilin Lu, Hongzhou Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title_full | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title_fullStr | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title_full_unstemmed | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title_short | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
title_sort | case report: histopathology and molecular pathology analysis on enteric tissue of a covid-19 patient |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097675/ https://www.ncbi.nlm.nih.gov/pubmed/33952310 http://dx.doi.org/10.1186/s13000-021-01082-7 |
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