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Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma
Glioblastomas (GBMs) are the most frequent and malignant type of brain tumor. It has been reported that progesterone (P4) regulates the progression of GBMs by modifying the expression of genes that promote cell proliferation, migration and invasion; however, it is not fully understood how these proc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097752/ https://www.ncbi.nlm.nih.gov/pubmed/33899118 http://dx.doi.org/10.3892/mmr.2021.12114 |
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author | Velázquez-Vázquez, Diana Elisa Del Moral-Morales, Aylin Cruz-Burgos, Jenie Marian Martínez-Martínez, Eduardo Rodríguez-Dorantes, Mauricio Camacho-Arroyo, Ignacio |
author_facet | Velázquez-Vázquez, Diana Elisa Del Moral-Morales, Aylin Cruz-Burgos, Jenie Marian Martínez-Martínez, Eduardo Rodríguez-Dorantes, Mauricio Camacho-Arroyo, Ignacio |
author_sort | Velázquez-Vázquez, Diana Elisa |
collection | PubMed |
description | Glioblastomas (GBMs) are the most frequent and malignant type of brain tumor. It has been reported that progesterone (P4) regulates the progression of GBMs by modifying the expression of genes that promote cell proliferation, migration and invasion; however, it is not fully understood how these processes are regulated. It is possible that P4 mediates some of these effects through changes in the microRNA (miRNA) expression profile in GBM cells. The present study investigated the effects of P4 on miRNAs expression profile in U-251MG cells derived from a human GBM. U-251MG cells were treated for 6 h with P4, RU486 (an antagonist of the intracellular progesterone receptor), the combined treatment (P4+RU486) and cyclodextrin (vehicle) and then a miRNA microarray analysis conducted. The expression analysis revealed a set of 190 miRNAs with differential expression in the treatments of P4, RU486 and P4+RU486 in respect to the vehicle and P4 in respect to P4+RU486, of which only 16 were exclusively regulated by P4. The possible mRNA targets of the miRNAs regulated by P4 could participate in the regulation of proliferation, cell cycle progression and cell migration of GBMs. The present study provided insight for understanding epigenetic modifications regulated by sex hormones involved in GBM progression, and for identifying potential therapeutic strategies for these brain tumors. |
format | Online Article Text |
id | pubmed-8097752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-80977522021-05-07 Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma Velázquez-Vázquez, Diana Elisa Del Moral-Morales, Aylin Cruz-Burgos, Jenie Marian Martínez-Martínez, Eduardo Rodríguez-Dorantes, Mauricio Camacho-Arroyo, Ignacio Mol Med Rep Articles Glioblastomas (GBMs) are the most frequent and malignant type of brain tumor. It has been reported that progesterone (P4) regulates the progression of GBMs by modifying the expression of genes that promote cell proliferation, migration and invasion; however, it is not fully understood how these processes are regulated. It is possible that P4 mediates some of these effects through changes in the microRNA (miRNA) expression profile in GBM cells. The present study investigated the effects of P4 on miRNAs expression profile in U-251MG cells derived from a human GBM. U-251MG cells were treated for 6 h with P4, RU486 (an antagonist of the intracellular progesterone receptor), the combined treatment (P4+RU486) and cyclodextrin (vehicle) and then a miRNA microarray analysis conducted. The expression analysis revealed a set of 190 miRNAs with differential expression in the treatments of P4, RU486 and P4+RU486 in respect to the vehicle and P4 in respect to P4+RU486, of which only 16 were exclusively regulated by P4. The possible mRNA targets of the miRNAs regulated by P4 could participate in the regulation of proliferation, cell cycle progression and cell migration of GBMs. The present study provided insight for understanding epigenetic modifications regulated by sex hormones involved in GBM progression, and for identifying potential therapeutic strategies for these brain tumors. D.A. Spandidos 2021-06 2021-04-22 /pmc/articles/PMC8097752/ /pubmed/33899118 http://dx.doi.org/10.3892/mmr.2021.12114 Text en Copyright: © Velázquez-Vázquez et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Velázquez-Vázquez, Diana Elisa Del Moral-Morales, Aylin Cruz-Burgos, Jenie Marian Martínez-Martínez, Eduardo Rodríguez-Dorantes, Mauricio Camacho-Arroyo, Ignacio Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title | Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title_full | Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title_fullStr | Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title_full_unstemmed | Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title_short | Expression analysis of progesterone-regulated miRNAs in cells derived from human glioblastoma |
title_sort | expression analysis of progesterone-regulated mirnas in cells derived from human glioblastoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097752/ https://www.ncbi.nlm.nih.gov/pubmed/33899118 http://dx.doi.org/10.3892/mmr.2021.12114 |
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