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Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program

During the COVID-19 pandemic, donor hematopoietic stem cell grafts are frequently cryopreserved to ensure the availability of graft before starting a conditioning regimen. However, the safety of cryopreservation has been controversial in unrelated hematopoietic stem cell transplantation (HSCT), espe...

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Autores principales: Kanda, Yoshinobu, Inoue, Masami, Uchida, Naoyuki, Onishi, Yasushi, Kamata, Reiko, Kotaki, Mika, Kobayashi, Ryoji, Tanaka, Junji, Fukuda, Takahiro, Fujii, Nobuharu, Miyamura, Koichi, Mori, Shin-Ichiro, Mori, Yasuo, Morishima, Yasuo, Yabe, Hiromasa, Kodera, Yoshihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098035/
https://www.ncbi.nlm.nih.gov/pubmed/33964514
http://dx.doi.org/10.1016/j.jtct.2021.04.022
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author Kanda, Yoshinobu
Inoue, Masami
Uchida, Naoyuki
Onishi, Yasushi
Kamata, Reiko
Kotaki, Mika
Kobayashi, Ryoji
Tanaka, Junji
Fukuda, Takahiro
Fujii, Nobuharu
Miyamura, Koichi
Mori, Shin-Ichiro
Mori, Yasuo
Morishima, Yasuo
Yabe, Hiromasa
Kodera, Yoshihisa
author_facet Kanda, Yoshinobu
Inoue, Masami
Uchida, Naoyuki
Onishi, Yasushi
Kamata, Reiko
Kotaki, Mika
Kobayashi, Ryoji
Tanaka, Junji
Fukuda, Takahiro
Fujii, Nobuharu
Miyamura, Koichi
Mori, Shin-Ichiro
Mori, Yasuo
Morishima, Yasuo
Yabe, Hiromasa
Kodera, Yoshihisa
author_sort Kanda, Yoshinobu
collection PubMed
description During the COVID-19 pandemic, donor hematopoietic stem cell grafts are frequently cryopreserved to ensure the availability of graft before starting a conditioning regimen. However, the safety of cryopreservation has been controversial in unrelated hematopoietic stem cell transplantation (HSCT), especially for bone marrow (BM) grafts. In addition, in unrelated HSCT, the effect of the time from harvest to cryopreservation of donor grafts required for the transportation of donor graft has not been fully clarified. In this study, we retrospectively analyzed the first 112 patients with available data who underwent cryopreserved unrelated blood and marrow transplantation through the Japan Marrow Donor Program during the COVID-19 pandemic. There were 112 patients, including 83 who received BM grafts and 29 who received peripheral blood stem cell (PBSC) grafts. The median time from stem cell harvest to cryopreservation was 9.9 hours (range, 2.6 to 44.0 hours), and the median time from cryopreservation to infusion was 231.2 hours. The incidence of neutrophil engraftment at day 28 after HSCT was 91.1%, and among 109 patients (excluding 3 patients with early death), all but 1 patient achieved neutrophil engraftment within 60 days after HSCT. The time to neutrophil engraftment and time to platelet engraftment were shorter in PBSC transplantation compared with BM transplantation (BMT), but the differences were not statistically significant (P = .064 and .18). Multivariate analysis among BM recipients revealed that a higher number of frozen nucleated cells and the absence of HLA mismatch were associated with faster neutrophil engraftment. The time to neutrophil engraftment after unrelated cryopreserved BMT was not different from that after unrelated BMT without cryopreservation. Our findings suggest that unrelated donor BM and PBSC grafts can be safely cryopreserved even after transit from the harvest center to the transplantation center. In the current COVID-19 pandemic, cryopreservation can be considered as an option while balancing the risks and benefits of the procedure.
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spelling pubmed-80980352021-05-05 Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program Kanda, Yoshinobu Inoue, Masami Uchida, Naoyuki Onishi, Yasushi Kamata, Reiko Kotaki, Mika Kobayashi, Ryoji Tanaka, Junji Fukuda, Takahiro Fujii, Nobuharu Miyamura, Koichi Mori, Shin-Ichiro Mori, Yasuo Morishima, Yasuo Yabe, Hiromasa Kodera, Yoshihisa Transplant Cell Ther Full Length Article During the COVID-19 pandemic, donor hematopoietic stem cell grafts are frequently cryopreserved to ensure the availability of graft before starting a conditioning regimen. However, the safety of cryopreservation has been controversial in unrelated hematopoietic stem cell transplantation (HSCT), especially for bone marrow (BM) grafts. In addition, in unrelated HSCT, the effect of the time from harvest to cryopreservation of donor grafts required for the transportation of donor graft has not been fully clarified. In this study, we retrospectively analyzed the first 112 patients with available data who underwent cryopreserved unrelated blood and marrow transplantation through the Japan Marrow Donor Program during the COVID-19 pandemic. There were 112 patients, including 83 who received BM grafts and 29 who received peripheral blood stem cell (PBSC) grafts. The median time from stem cell harvest to cryopreservation was 9.9 hours (range, 2.6 to 44.0 hours), and the median time from cryopreservation to infusion was 231.2 hours. The incidence of neutrophil engraftment at day 28 after HSCT was 91.1%, and among 109 patients (excluding 3 patients with early death), all but 1 patient achieved neutrophil engraftment within 60 days after HSCT. The time to neutrophil engraftment and time to platelet engraftment were shorter in PBSC transplantation compared with BM transplantation (BMT), but the differences were not statistically significant (P = .064 and .18). Multivariate analysis among BM recipients revealed that a higher number of frozen nucleated cells and the absence of HLA mismatch were associated with faster neutrophil engraftment. The time to neutrophil engraftment after unrelated cryopreserved BMT was not different from that after unrelated BMT without cryopreservation. Our findings suggest that unrelated donor BM and PBSC grafts can be safely cryopreserved even after transit from the harvest center to the transplantation center. In the current COVID-19 pandemic, cryopreservation can be considered as an option while balancing the risks and benefits of the procedure. The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. 2021-08 2021-05-05 /pmc/articles/PMC8098035/ /pubmed/33964514 http://dx.doi.org/10.1016/j.jtct.2021.04.022 Text en © 2021 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Full Length Article
Kanda, Yoshinobu
Inoue, Masami
Uchida, Naoyuki
Onishi, Yasushi
Kamata, Reiko
Kotaki, Mika
Kobayashi, Ryoji
Tanaka, Junji
Fukuda, Takahiro
Fujii, Nobuharu
Miyamura, Koichi
Mori, Shin-Ichiro
Mori, Yasuo
Morishima, Yasuo
Yabe, Hiromasa
Kodera, Yoshihisa
Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title_full Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title_fullStr Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title_full_unstemmed Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title_short Cryopreservation of Unrelated Hematopoietic Stem Cells from a Blood and Marrow Donor Bank During the COVID-19 Pandemic: A Nationwide Survey by the Japan Marrow Donor Program
title_sort cryopreservation of unrelated hematopoietic stem cells from a blood and marrow donor bank during the covid-19 pandemic: a nationwide survey by the japan marrow donor program
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098035/
https://www.ncbi.nlm.nih.gov/pubmed/33964514
http://dx.doi.org/10.1016/j.jtct.2021.04.022
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