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Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism

BACKGROUND: Alcoholic liver disease is caused as a result of chronic alcohol consumption. In this study, we used an alcoholic liver injury mouse model to investigate the effect of fucoidan on ethanol-induced liver injury and steatosis and the underlying mechanisms. METHODS: All mice were randomly di...

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Autores principales: Xue, Meilan, Liang, Hui, Zhou, Zhitong, Liu, Ying, He, Xinjia, Zhang, Zheng, Sun, Ting, Yang, Jia, Qin, Yimin, Qin, Kunpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Academia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098649/
https://www.ncbi.nlm.nih.gov/pubmed/33994911
http://dx.doi.org/10.29219/fnr.v65.5384
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author Xue, Meilan
Liang, Hui
Zhou, Zhitong
Liu, Ying
He, Xinjia
Zhang, Zheng
Sun, Ting
Yang, Jia
Qin, Yimin
Qin, Kunpeng
author_facet Xue, Meilan
Liang, Hui
Zhou, Zhitong
Liu, Ying
He, Xinjia
Zhang, Zheng
Sun, Ting
Yang, Jia
Qin, Yimin
Qin, Kunpeng
author_sort Xue, Meilan
collection PubMed
description BACKGROUND: Alcoholic liver disease is caused as a result of chronic alcohol consumption. In this study, we used an alcoholic liver injury mouse model to investigate the effect of fucoidan on ethanol-induced liver injury and steatosis and the underlying mechanisms. METHODS: All mice were randomly divided into four groups: 1) control group, 2) model group, 3) diammonium glycyrrhizinate treatment group (200 mg/kg body weight), and 4) fucoidan treatment group (300 mg/kg body weight). Administration of ethanol for 8 weeks induced liver injury and steatosis in mice. RESULTS: Fucoidan treatment decreased serum alanine aminotransferase activity, serum total cholesterol levels, and hepatic triglyceride levels, and improved the morphology of hepatic cells. Fucoidan treatment upregulated the expression of AMPKα1, SIRT1, and PGC-1α and inhibited the expression of ChREBP and HNF-1α. The levels of hepatic IL-6 and IL-18 were significantly decreased in the fucoidan group. Further, the levels of cytochrome P450-2E1 (CYP2E1), glucose-regulated protein (GRP) 78, and 3-nitrotyrosine (3-NT) in hepatic tissues were reduced in the fucoidan group as compared to the model group. Fucoidan significantly reversed the reduction of ileac Farnesoid X receptor (FXR) and fibroblast growth factor 15 (FGF15) levels induced by alcohol-feeding and reduced CYP7A1 (cholesterol 7α-hydroxylase) expression and total bile acid levels in the liver tissue. In addition, fucoidan regulated the structure of gut flora, with increased abundance of Prevotella and decreased abundance of Paraprevotella and Romboutsia as detected by 16S rDNA high-throughput sequencing. CONCLUSION: Fucoidan inhibited alcohol-induced steatosis and disorders of bile acid metabolism in mice through the AMPKα1/SIRT1 pathway and the gut microbiota–bile acid–liver axis and protected against alcohol-induced liver injury in vivo.
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spelling pubmed-80986492021-05-14 Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism Xue, Meilan Liang, Hui Zhou, Zhitong Liu, Ying He, Xinjia Zhang, Zheng Sun, Ting Yang, Jia Qin, Yimin Qin, Kunpeng Food Nutr Res Original Article BACKGROUND: Alcoholic liver disease is caused as a result of chronic alcohol consumption. In this study, we used an alcoholic liver injury mouse model to investigate the effect of fucoidan on ethanol-induced liver injury and steatosis and the underlying mechanisms. METHODS: All mice were randomly divided into four groups: 1) control group, 2) model group, 3) diammonium glycyrrhizinate treatment group (200 mg/kg body weight), and 4) fucoidan treatment group (300 mg/kg body weight). Administration of ethanol for 8 weeks induced liver injury and steatosis in mice. RESULTS: Fucoidan treatment decreased serum alanine aminotransferase activity, serum total cholesterol levels, and hepatic triglyceride levels, and improved the morphology of hepatic cells. Fucoidan treatment upregulated the expression of AMPKα1, SIRT1, and PGC-1α and inhibited the expression of ChREBP and HNF-1α. The levels of hepatic IL-6 and IL-18 were significantly decreased in the fucoidan group. Further, the levels of cytochrome P450-2E1 (CYP2E1), glucose-regulated protein (GRP) 78, and 3-nitrotyrosine (3-NT) in hepatic tissues were reduced in the fucoidan group as compared to the model group. Fucoidan significantly reversed the reduction of ileac Farnesoid X receptor (FXR) and fibroblast growth factor 15 (FGF15) levels induced by alcohol-feeding and reduced CYP7A1 (cholesterol 7α-hydroxylase) expression and total bile acid levels in the liver tissue. In addition, fucoidan regulated the structure of gut flora, with increased abundance of Prevotella and decreased abundance of Paraprevotella and Romboutsia as detected by 16S rDNA high-throughput sequencing. CONCLUSION: Fucoidan inhibited alcohol-induced steatosis and disorders of bile acid metabolism in mice through the AMPKα1/SIRT1 pathway and the gut microbiota–bile acid–liver axis and protected against alcohol-induced liver injury in vivo. Open Academia 2021-04-20 /pmc/articles/PMC8098649/ /pubmed/33994911 http://dx.doi.org/10.29219/fnr.v65.5384 Text en © 2021 Meilan Xue et al. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
spellingShingle Original Article
Xue, Meilan
Liang, Hui
Zhou, Zhitong
Liu, Ying
He, Xinjia
Zhang, Zheng
Sun, Ting
Yang, Jia
Qin, Yimin
Qin, Kunpeng
Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title_full Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title_fullStr Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title_full_unstemmed Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title_short Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
title_sort effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098649/
https://www.ncbi.nlm.nih.gov/pubmed/33994911
http://dx.doi.org/10.29219/fnr.v65.5384
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