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Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus

Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O....

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Autores principales: Park, Sung-Moo, Gu, Min Jeong, Ju, Young-Jun, Cheon, In Su, Hwang, Kyu-Jam, Gill, Byoungchul, Shim, Byoung-Shik, Jeong, Hang-Jin, Son, Young Min, Choi, Sangho, Jeung, Woonhee, Han, Seung Hyun, Chu, Hyuk, Yun, Cheol-Heui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099613/
https://www.ncbi.nlm.nih.gov/pubmed/33996170
http://dx.doi.org/10.4110/in.2021.21.e14
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author Park, Sung-Moo
Gu, Min Jeong
Ju, Young-Jun
Cheon, In Su
Hwang, Kyu-Jam
Gill, Byoungchul
Shim, Byoung-Shik
Jeong, Hang-Jin
Son, Young Min
Choi, Sangho
Jeung, Woonhee
Han, Seung Hyun
Chu, Hyuk
Yun, Cheol-Heui
author_facet Park, Sung-Moo
Gu, Min Jeong
Ju, Young-Jun
Cheon, In Su
Hwang, Kyu-Jam
Gill, Byoungchul
Shim, Byoung-Shik
Jeong, Hang-Jin
Son, Young Min
Choi, Sangho
Jeung, Woonhee
Han, Seung Hyun
Chu, Hyuk
Yun, Cheol-Heui
author_sort Park, Sung-Moo
collection PubMed
description Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O. tsutsugamushi (OMPOT) would induce a protective immunity against O. tsutsugamushi infection. It was particular interest that when mice were infected with O. tsutsugamushi, the bacteria disseminated into the lungs, causing pneumonia. The i.n. vaccination with OMPOT induced IgG responses in serum and bronchoalveolar lavage (BAL) fluid. The anti-O. tsutsugamushi IgA Abs in BAL fluid after the vaccination showed a high correlation of the protection against O. tsutsugamushi. The vaccination induced strong Ag-specific Th1 and Th17 responses in the both spleen and lungs. In conclusion, the current study demonstrated that i.n. vaccination with OMPOT elicited protective immunity against scrub typhus in mouse with O. tsutsugamushi infection causing subsequent pneumonia.
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spelling pubmed-80996132021-05-14 Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus Park, Sung-Moo Gu, Min Jeong Ju, Young-Jun Cheon, In Su Hwang, Kyu-Jam Gill, Byoungchul Shim, Byoung-Shik Jeong, Hang-Jin Son, Young Min Choi, Sangho Jeung, Woonhee Han, Seung Hyun Chu, Hyuk Yun, Cheol-Heui Immune Netw Original Article Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O. tsutsugamushi (OMPOT) would induce a protective immunity against O. tsutsugamushi infection. It was particular interest that when mice were infected with O. tsutsugamushi, the bacteria disseminated into the lungs, causing pneumonia. The i.n. vaccination with OMPOT induced IgG responses in serum and bronchoalveolar lavage (BAL) fluid. The anti-O. tsutsugamushi IgA Abs in BAL fluid after the vaccination showed a high correlation of the protection against O. tsutsugamushi. The vaccination induced strong Ag-specific Th1 and Th17 responses in the both spleen and lungs. In conclusion, the current study demonstrated that i.n. vaccination with OMPOT elicited protective immunity against scrub typhus in mouse with O. tsutsugamushi infection causing subsequent pneumonia. The Korean Association of Immunologists 2020-12-03 /pmc/articles/PMC8099613/ /pubmed/33996170 http://dx.doi.org/10.4110/in.2021.21.e14 Text en Copyright © 2021. The Korean Association of Immunologists https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Sung-Moo
Gu, Min Jeong
Ju, Young-Jun
Cheon, In Su
Hwang, Kyu-Jam
Gill, Byoungchul
Shim, Byoung-Shik
Jeong, Hang-Jin
Son, Young Min
Choi, Sangho
Jeung, Woonhee
Han, Seung Hyun
Chu, Hyuk
Yun, Cheol-Heui
Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title_full Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title_fullStr Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title_full_unstemmed Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title_short Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus
title_sort intranasal vaccination with outer-membrane protein of orientia tsutsugamushi induces protective immunity against scrub typhus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099613/
https://www.ncbi.nlm.nih.gov/pubmed/33996170
http://dx.doi.org/10.4110/in.2021.21.e14
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