Cargando…
Estrogen modulates epithelial progenitor cells in rat vagina
PURPOSE: The expression of epithelial progenitor cells (EPCs) in rat vagina was recently reported. The aims were to investigate the effects of estrogen on vaginal EPCs in the oophorectomized female rat model. MATERIALS AND METHODS: Female Sprague-Dawley rats (230–240 g, n=30) were divided into 3 gro...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Urological Association
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100018/ https://www.ncbi.nlm.nih.gov/pubmed/33834644 http://dx.doi.org/10.4111/icu.20200513 |
_version_ | 1783688689311809536 |
---|---|
author | Chung, Ho Seok Lee, Hyun-Suk Park, Kwangsung |
author_facet | Chung, Ho Seok Lee, Hyun-Suk Park, Kwangsung |
author_sort | Chung, Ho Seok |
collection | PubMed |
description | PURPOSE: The expression of epithelial progenitor cells (EPCs) in rat vagina was recently reported. The aims were to investigate the effects of estrogen on vaginal EPCs in the oophorectomized female rat model. MATERIALS AND METHODS: Female Sprague-Dawley rats (230–240 g, n=30) were divided into 3 groups: control (n=10), bilateral oophorectomy (OVX, n=10), and bilateral OVX followed by subcutaneous injections of 17β-estradiol (50 µg/kg/day, n=10). After 4 weeks, the expression of EPC-specific markers (CD44, estrogen receptor alpha [ERα], and progesterone receptor) were evaluated by immunohistochemistry and Western blot. RESULTS: The CD44/ERα double-labeled cells were mainly expressed in basal cell layers and suprabasal layers as shown by confocal immunofluorescence. Confocal microscopy revealed that the number of CD44+/ERα+ cells decreased in the OVX group compared with the controls but was similar to control levels in rats receiving estrogen replacements. The protein expression of CD44 and ERα decreased after OVX and was restored to control levels after estrogen supplementation. CONCLUSIONS: Markers of EPCs were expressed in the vagina, and the expression of resident EPCs was regulated by estrogen. These findings imply that resident EPCs may have an important role in the regeneration of vaginal mucosa by estrogen replacement. |
format | Online Article Text |
id | pubmed-8100018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Urological Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-81000182021-05-14 Estrogen modulates epithelial progenitor cells in rat vagina Chung, Ho Seok Lee, Hyun-Suk Park, Kwangsung Investig Clin Urol Original Article PURPOSE: The expression of epithelial progenitor cells (EPCs) in rat vagina was recently reported. The aims were to investigate the effects of estrogen on vaginal EPCs in the oophorectomized female rat model. MATERIALS AND METHODS: Female Sprague-Dawley rats (230–240 g, n=30) were divided into 3 groups: control (n=10), bilateral oophorectomy (OVX, n=10), and bilateral OVX followed by subcutaneous injections of 17β-estradiol (50 µg/kg/day, n=10). After 4 weeks, the expression of EPC-specific markers (CD44, estrogen receptor alpha [ERα], and progesterone receptor) were evaluated by immunohistochemistry and Western blot. RESULTS: The CD44/ERα double-labeled cells were mainly expressed in basal cell layers and suprabasal layers as shown by confocal immunofluorescence. Confocal microscopy revealed that the number of CD44+/ERα+ cells decreased in the OVX group compared with the controls but was similar to control levels in rats receiving estrogen replacements. The protein expression of CD44 and ERα decreased after OVX and was restored to control levels after estrogen supplementation. CONCLUSIONS: Markers of EPCs were expressed in the vagina, and the expression of resident EPCs was regulated by estrogen. These findings imply that resident EPCs may have an important role in the regeneration of vaginal mucosa by estrogen replacement. The Korean Urological Association 2021-05 2021-04-02 /pmc/articles/PMC8100018/ /pubmed/33834644 http://dx.doi.org/10.4111/icu.20200513 Text en © The Korean Urological Association, 2021 https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Chung, Ho Seok Lee, Hyun-Suk Park, Kwangsung Estrogen modulates epithelial progenitor cells in rat vagina |
title | Estrogen modulates epithelial progenitor cells in rat vagina |
title_full | Estrogen modulates epithelial progenitor cells in rat vagina |
title_fullStr | Estrogen modulates epithelial progenitor cells in rat vagina |
title_full_unstemmed | Estrogen modulates epithelial progenitor cells in rat vagina |
title_short | Estrogen modulates epithelial progenitor cells in rat vagina |
title_sort | estrogen modulates epithelial progenitor cells in rat vagina |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100018/ https://www.ncbi.nlm.nih.gov/pubmed/33834644 http://dx.doi.org/10.4111/icu.20200513 |
work_keys_str_mv | AT chunghoseok estrogenmodulatesepithelialprogenitorcellsinratvagina AT leehyunsuk estrogenmodulatesepithelialprogenitorcellsinratvagina AT parkkwangsung estrogenmodulatesepithelialprogenitorcellsinratvagina |