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An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants

The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain...

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Autores principales: Vargas Hoyos, Harold Alexander, Chiaramonte, Josiane Barros, Barbosa-Casteliani, Ana Gabriele, Fernandez Morais, Jorge, Perez-Jaramillo, Juan Esteban, Nobre Santos, Suikinai, Nascimento Queiroz, Sonia Claudia, Soares Melo, Itamar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100043/
https://www.ncbi.nlm.nih.gov/pubmed/33968908
http://dx.doi.org/10.3389/fbioe.2021.579906
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author Vargas Hoyos, Harold Alexander
Chiaramonte, Josiane Barros
Barbosa-Casteliani, Ana Gabriele
Fernandez Morais, Jorge
Perez-Jaramillo, Juan Esteban
Nobre Santos, Suikinai
Nascimento Queiroz, Sonia Claudia
Soares Melo, Itamar
author_facet Vargas Hoyos, Harold Alexander
Chiaramonte, Josiane Barros
Barbosa-Casteliani, Ana Gabriele
Fernandez Morais, Jorge
Perez-Jaramillo, Juan Esteban
Nobre Santos, Suikinai
Nascimento Queiroz, Sonia Claudia
Soares Melo, Itamar
author_sort Vargas Hoyos, Harold Alexander
collection PubMed
description The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain 3AS4) from wheat rhizospheres of crops cultivated in the Cerrado biome. Strain 3AS4 was identified as belonging to the genus Streptomyces and had phosphorus mobilization ability, mineralizing approximately 410 μg ml(–1) from phytate, 300 μg ml(–1) from calcium phosphate, and 200 μg ml(–1) from rock phosphate. The analysis of the actinobacteria crude extract by spectrometric techniques revealed the presence of gluconic and 2-ketogluconic acid, and a greenhouse experiment was carried out to evaluate its plant growth promotion activity in soybean. Soil in its natural condition (with no phosphorus addition), 40 kg ha(–1) rock phosphate from Bayovar (RP) added to soil, and triple super phosphate (SPT) added to soil were used. Significant differences in plant height were observed at 6 weeks when the plants were inoculated with the 3AS4 strain. The growth of inoculated plants in natural condition was promoted in 17% compared with the RP and SPT non-inoculated conditions, suggesting that inoculation can enable plants to grow with lower chemical P fertilizers. In the plants that were inoculated with the 3AS4 strain in the RP condition, the plant height increased by approximately 80% and the shoot:root ratio was approximately 30% higher compared to control conditions (non-inoculated plants in natural conditions). 3AS4 has P-solubilizing potential and can be exploited as an inoculant for soybean cultivation. These results suggest that this actinobacterium is a valuable resource for sustainable agriculture and will allow the reduction of phosphate fertilization in the future.
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spelling pubmed-81000432021-05-07 An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants Vargas Hoyos, Harold Alexander Chiaramonte, Josiane Barros Barbosa-Casteliani, Ana Gabriele Fernandez Morais, Jorge Perez-Jaramillo, Juan Esteban Nobre Santos, Suikinai Nascimento Queiroz, Sonia Claudia Soares Melo, Itamar Front Bioeng Biotechnol Bioengineering and Biotechnology The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain 3AS4) from wheat rhizospheres of crops cultivated in the Cerrado biome. Strain 3AS4 was identified as belonging to the genus Streptomyces and had phosphorus mobilization ability, mineralizing approximately 410 μg ml(–1) from phytate, 300 μg ml(–1) from calcium phosphate, and 200 μg ml(–1) from rock phosphate. The analysis of the actinobacteria crude extract by spectrometric techniques revealed the presence of gluconic and 2-ketogluconic acid, and a greenhouse experiment was carried out to evaluate its plant growth promotion activity in soybean. Soil in its natural condition (with no phosphorus addition), 40 kg ha(–1) rock phosphate from Bayovar (RP) added to soil, and triple super phosphate (SPT) added to soil were used. Significant differences in plant height were observed at 6 weeks when the plants were inoculated with the 3AS4 strain. The growth of inoculated plants in natural condition was promoted in 17% compared with the RP and SPT non-inoculated conditions, suggesting that inoculation can enable plants to grow with lower chemical P fertilizers. In the plants that were inoculated with the 3AS4 strain in the RP condition, the plant height increased by approximately 80% and the shoot:root ratio was approximately 30% higher compared to control conditions (non-inoculated plants in natural conditions). 3AS4 has P-solubilizing potential and can be exploited as an inoculant for soybean cultivation. These results suggest that this actinobacterium is a valuable resource for sustainable agriculture and will allow the reduction of phosphate fertilization in the future. Frontiers Media S.A. 2021-04-22 /pmc/articles/PMC8100043/ /pubmed/33968908 http://dx.doi.org/10.3389/fbioe.2021.579906 Text en Copyright © 2021 Vargas Hoyos, Chiaramonte, Barbosa-Casteliani, Fernandez Morais, Perez-Jaramillo, Nobre Santos, Nascimento Queiroz and Soares Melo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Vargas Hoyos, Harold Alexander
Chiaramonte, Josiane Barros
Barbosa-Casteliani, Ana Gabriele
Fernandez Morais, Jorge
Perez-Jaramillo, Juan Esteban
Nobre Santos, Suikinai
Nascimento Queiroz, Sonia Claudia
Soares Melo, Itamar
An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title_full An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title_fullStr An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title_full_unstemmed An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title_short An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants
title_sort actinobacterium strain from soil of cerrado promotes phosphorus solubilization and plant growth in soybean plants
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100043/
https://www.ncbi.nlm.nih.gov/pubmed/33968908
http://dx.doi.org/10.3389/fbioe.2021.579906
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