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Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer

Lung cancer is a highly heterogeneous disease. Cancer cells and cells within the tumor microenvironment together determine disease progression, as well as response to or escape from treatment. To map the cell type-specific transcriptome landscape of cancer cells and their tumor microenvironment in a...

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Autores principales: Wu, Fengying, Fan, Jue, He, Yayi, Xiong, Anwen, Yu, Jia, Li, Yixin, Zhang, Yan, Zhao, Wencheng, Zhou, Fei, Li, Wei, Zhang, Jie, Zhang, Xiaosheng, Qiao, Meng, Gao, Guanghui, Chen, Shanhao, Chen, Xiaoxia, Li, Xuefei, Hou, Likun, Wu, Chunyan, Su, Chunxia, Ren, Shengxiang, Odenthal, Margarete, Buettner, Reinhard, Fang, Nan, Zhou, Caicun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100173/
https://www.ncbi.nlm.nih.gov/pubmed/33953163
http://dx.doi.org/10.1038/s41467-021-22801-0
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author Wu, Fengying
Fan, Jue
He, Yayi
Xiong, Anwen
Yu, Jia
Li, Yixin
Zhang, Yan
Zhao, Wencheng
Zhou, Fei
Li, Wei
Zhang, Jie
Zhang, Xiaosheng
Qiao, Meng
Gao, Guanghui
Chen, Shanhao
Chen, Xiaoxia
Li, Xuefei
Hou, Likun
Wu, Chunyan
Su, Chunxia
Ren, Shengxiang
Odenthal, Margarete
Buettner, Reinhard
Fang, Nan
Zhou, Caicun
author_facet Wu, Fengying
Fan, Jue
He, Yayi
Xiong, Anwen
Yu, Jia
Li, Yixin
Zhang, Yan
Zhao, Wencheng
Zhou, Fei
Li, Wei
Zhang, Jie
Zhang, Xiaosheng
Qiao, Meng
Gao, Guanghui
Chen, Shanhao
Chen, Xiaoxia
Li, Xuefei
Hou, Likun
Wu, Chunyan
Su, Chunxia
Ren, Shengxiang
Odenthal, Margarete
Buettner, Reinhard
Fang, Nan
Zhou, Caicun
author_sort Wu, Fengying
collection PubMed
description Lung cancer is a highly heterogeneous disease. Cancer cells and cells within the tumor microenvironment together determine disease progression, as well as response to or escape from treatment. To map the cell type-specific transcriptome landscape of cancer cells and their tumor microenvironment in advanced non-small cell lung cancer (NSCLC), we analyze 42 tissue biopsy samples from stage III/IV NSCLC patients by single cell RNA sequencing and present the large scale, single cell resolution profiles of advanced NSCLCs. In addition to cell types described in previous single cell studies of early stage lung cancer, we are able to identify rare cell types in tumors such as follicular dendritic cells and T helper 17 cells. Tumors from different patients display large heterogeneity in cellular composition, chromosomal structure, developmental trajectory, intercellular signaling network and phenotype dominance. Our study also reveals a correlation of tumor heterogeneity with tumor associated neutrophils, which might help to shed light on their function in NSCLC.
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spelling pubmed-81001732021-05-11 Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer Wu, Fengying Fan, Jue He, Yayi Xiong, Anwen Yu, Jia Li, Yixin Zhang, Yan Zhao, Wencheng Zhou, Fei Li, Wei Zhang, Jie Zhang, Xiaosheng Qiao, Meng Gao, Guanghui Chen, Shanhao Chen, Xiaoxia Li, Xuefei Hou, Likun Wu, Chunyan Su, Chunxia Ren, Shengxiang Odenthal, Margarete Buettner, Reinhard Fang, Nan Zhou, Caicun Nat Commun Article Lung cancer is a highly heterogeneous disease. Cancer cells and cells within the tumor microenvironment together determine disease progression, as well as response to or escape from treatment. To map the cell type-specific transcriptome landscape of cancer cells and their tumor microenvironment in advanced non-small cell lung cancer (NSCLC), we analyze 42 tissue biopsy samples from stage III/IV NSCLC patients by single cell RNA sequencing and present the large scale, single cell resolution profiles of advanced NSCLCs. In addition to cell types described in previous single cell studies of early stage lung cancer, we are able to identify rare cell types in tumors such as follicular dendritic cells and T helper 17 cells. Tumors from different patients display large heterogeneity in cellular composition, chromosomal structure, developmental trajectory, intercellular signaling network and phenotype dominance. Our study also reveals a correlation of tumor heterogeneity with tumor associated neutrophils, which might help to shed light on their function in NSCLC. Nature Publishing Group UK 2021-05-05 /pmc/articles/PMC8100173/ /pubmed/33953163 http://dx.doi.org/10.1038/s41467-021-22801-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wu, Fengying
Fan, Jue
He, Yayi
Xiong, Anwen
Yu, Jia
Li, Yixin
Zhang, Yan
Zhao, Wencheng
Zhou, Fei
Li, Wei
Zhang, Jie
Zhang, Xiaosheng
Qiao, Meng
Gao, Guanghui
Chen, Shanhao
Chen, Xiaoxia
Li, Xuefei
Hou, Likun
Wu, Chunyan
Su, Chunxia
Ren, Shengxiang
Odenthal, Margarete
Buettner, Reinhard
Fang, Nan
Zhou, Caicun
Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title_full Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title_fullStr Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title_full_unstemmed Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title_short Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
title_sort single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100173/
https://www.ncbi.nlm.nih.gov/pubmed/33953163
http://dx.doi.org/10.1038/s41467-021-22801-0
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