Cargando…

Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking

BACKGROUND: Asthma is a chronic inflammatory disease characterized by Th2-predominant inflammation and airway remodeling. Modified Guo Min decoction (MGMD) has been an extensive practical strategy for allergic disorders in China. Although its potential anti-asthmatic activity has been reported, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Guishu, Zhou, Bo, Wang, Zheyi, Meng, Yufeng, Liu, Yaqian, Yao, Xiaoqin, Feng, Cuiling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100455/
https://www.ncbi.nlm.nih.gov/pubmed/33968984
http://dx.doi.org/10.3389/fmolb.2021.644561
_version_ 1783688793185845248
author Wang, Guishu
Zhou, Bo
Wang, Zheyi
Meng, Yufeng
Liu, Yaqian
Yao, Xiaoqin
Feng, Cuiling
author_facet Wang, Guishu
Zhou, Bo
Wang, Zheyi
Meng, Yufeng
Liu, Yaqian
Yao, Xiaoqin
Feng, Cuiling
author_sort Wang, Guishu
collection PubMed
description BACKGROUND: Asthma is a chronic inflammatory disease characterized by Th2-predominant inflammation and airway remodeling. Modified Guo Min decoction (MGMD) has been an extensive practical strategy for allergic disorders in China. Although its potential anti-asthmatic activity has been reported, the exact mechanism of action of MGMD in asthma remains unexplored. METHODS: Network pharmacology approach was employed to predict the active components, potential targets, and molecular mechanism of MGMD for asthma treatment, including drug-likeness evaluation, oral bioavailability prediction, protein–protein interaction (PPI) network construction and analysis, Gene Ontology (GO) terms, and Reactome pathway annotation. Molecular docking was carried out to investigate interactions between active compounds and potential targets. RESULTS: A total of 92 active compounds and 72 anti-asthma targets of MGMD were selected for analysis. The GO enrichment analysis results indicated that the anti-asthmatic targets of MGMD mainly participate in inflammatory and in airway remolding processes. The Reactome pathway analysis showed that MGMD prevents asthma mainly through regulation of the IL-4 and IL-13 signaling and the specialized pro-resolving mediators (SPMs) biosynthesis. Molecular docking results suggest that each bioactive compounds (quercetin, wogonin, luteolin, naringenin, and kaempferol) is capable to bind with STAT3, PTGS2, JUN, VEGFA, EGFR, and ALOX5. CONCLUSION: This study revealed the active ingredients and potential molecular mechanism by which MGMD treatment is effective against airway inflammation and remodeling in asthma through regulating IL-4 and IL-13 signaling and SPMs biosynthesis.
format Online
Article
Text
id pubmed-8100455
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81004552021-05-07 Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking Wang, Guishu Zhou, Bo Wang, Zheyi Meng, Yufeng Liu, Yaqian Yao, Xiaoqin Feng, Cuiling Front Mol Biosci Molecular Biosciences BACKGROUND: Asthma is a chronic inflammatory disease characterized by Th2-predominant inflammation and airway remodeling. Modified Guo Min decoction (MGMD) has been an extensive practical strategy for allergic disorders in China. Although its potential anti-asthmatic activity has been reported, the exact mechanism of action of MGMD in asthma remains unexplored. METHODS: Network pharmacology approach was employed to predict the active components, potential targets, and molecular mechanism of MGMD for asthma treatment, including drug-likeness evaluation, oral bioavailability prediction, protein–protein interaction (PPI) network construction and analysis, Gene Ontology (GO) terms, and Reactome pathway annotation. Molecular docking was carried out to investigate interactions between active compounds and potential targets. RESULTS: A total of 92 active compounds and 72 anti-asthma targets of MGMD were selected for analysis. The GO enrichment analysis results indicated that the anti-asthmatic targets of MGMD mainly participate in inflammatory and in airway remolding processes. The Reactome pathway analysis showed that MGMD prevents asthma mainly through regulation of the IL-4 and IL-13 signaling and the specialized pro-resolving mediators (SPMs) biosynthesis. Molecular docking results suggest that each bioactive compounds (quercetin, wogonin, luteolin, naringenin, and kaempferol) is capable to bind with STAT3, PTGS2, JUN, VEGFA, EGFR, and ALOX5. CONCLUSION: This study revealed the active ingredients and potential molecular mechanism by which MGMD treatment is effective against airway inflammation and remodeling in asthma through regulating IL-4 and IL-13 signaling and SPMs biosynthesis. Frontiers Media S.A. 2021-04-22 /pmc/articles/PMC8100455/ /pubmed/33968984 http://dx.doi.org/10.3389/fmolb.2021.644561 Text en Copyright © 2021 Wang, Zhou, Wang, Meng, Liu, Yao and Feng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Wang, Guishu
Zhou, Bo
Wang, Zheyi
Meng, Yufeng
Liu, Yaqian
Yao, Xiaoqin
Feng, Cuiling
Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title_full Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title_fullStr Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title_full_unstemmed Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title_short Pharmacological Mechanisms Underlying the Anti-asthmatic Effects of Modified Guomin Decoction Determined by Network Pharmacology and Molecular Docking
title_sort pharmacological mechanisms underlying the anti-asthmatic effects of modified guomin decoction determined by network pharmacology and molecular docking
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100455/
https://www.ncbi.nlm.nih.gov/pubmed/33968984
http://dx.doi.org/10.3389/fmolb.2021.644561
work_keys_str_mv AT wangguishu pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT zhoubo pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT wangzheyi pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT mengyufeng pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT liuyaqian pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT yaoxiaoqin pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking
AT fengcuiling pharmacologicalmechanismsunderlyingtheantiasthmaticeffectsofmodifiedguomindecoctiondeterminedbynetworkpharmacologyandmoleculardocking