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Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality

A large proportion of infertility and miscarriage causes are unknown. One potential cause is a defective sperm centriole, a subcellular structure essential for sperm motility and embryonic development. Yet, the extent to which centriolar maladies contribute to male infertility is unknown due to the...

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Autores principales: Turner, Katerina A., Fishman, Emily L., Asadullah, Mariam, Ott, Brooke, Dusza, Patrick, Shah, Tariq A., Sindhwani, Puneet, Nadiminty, Nagalakshmi, Molinari, Emanuela, Patrizio, Pasquale, Saltzman, Barbara S., Avidor-Reiss, Tomer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100587/
https://www.ncbi.nlm.nih.gov/pubmed/33968935
http://dx.doi.org/10.3389/fcell.2021.658891
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author Turner, Katerina A.
Fishman, Emily L.
Asadullah, Mariam
Ott, Brooke
Dusza, Patrick
Shah, Tariq A.
Sindhwani, Puneet
Nadiminty, Nagalakshmi
Molinari, Emanuela
Patrizio, Pasquale
Saltzman, Barbara S.
Avidor-Reiss, Tomer
author_facet Turner, Katerina A.
Fishman, Emily L.
Asadullah, Mariam
Ott, Brooke
Dusza, Patrick
Shah, Tariq A.
Sindhwani, Puneet
Nadiminty, Nagalakshmi
Molinari, Emanuela
Patrizio, Pasquale
Saltzman, Barbara S.
Avidor-Reiss, Tomer
author_sort Turner, Katerina A.
collection PubMed
description A large proportion of infertility and miscarriage causes are unknown. One potential cause is a defective sperm centriole, a subcellular structure essential for sperm motility and embryonic development. Yet, the extent to which centriolar maladies contribute to male infertility is unknown due to the lack of a convenient way to assess centriole quality. We developed a robust, location-based, ratiometric assay to overcome this roadblock, the Fluorescence-based Ratiometric Assessment of Centrioles (FRAC). We performed a case series study with semen samples from 33 patients, separated using differential gradient centrifugation into higher-grade (pellet) and lower-grade (interface) sperm fractions. Using a reference population of higher-grade sperm from infertile men with morphologically standard sperm, we found that 79% of higher-grade sperm of infertile men with substandard sperm morphology have suboptimal centrioles (P = 0.0005). Moreover, tubulin labeling of the sperm distal centriole correlates negatively with age (P = 0.004, R = −0.66). These findings suggest that FRAC is a sensitive method and that patient age and sperm morphology are associated with centriole quality.
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spelling pubmed-81005872021-05-07 Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality Turner, Katerina A. Fishman, Emily L. Asadullah, Mariam Ott, Brooke Dusza, Patrick Shah, Tariq A. Sindhwani, Puneet Nadiminty, Nagalakshmi Molinari, Emanuela Patrizio, Pasquale Saltzman, Barbara S. Avidor-Reiss, Tomer Front Cell Dev Biol Cell and Developmental Biology A large proportion of infertility and miscarriage causes are unknown. One potential cause is a defective sperm centriole, a subcellular structure essential for sperm motility and embryonic development. Yet, the extent to which centriolar maladies contribute to male infertility is unknown due to the lack of a convenient way to assess centriole quality. We developed a robust, location-based, ratiometric assay to overcome this roadblock, the Fluorescence-based Ratiometric Assessment of Centrioles (FRAC). We performed a case series study with semen samples from 33 patients, separated using differential gradient centrifugation into higher-grade (pellet) and lower-grade (interface) sperm fractions. Using a reference population of higher-grade sperm from infertile men with morphologically standard sperm, we found that 79% of higher-grade sperm of infertile men with substandard sperm morphology have suboptimal centrioles (P = 0.0005). Moreover, tubulin labeling of the sperm distal centriole correlates negatively with age (P = 0.004, R = −0.66). These findings suggest that FRAC is a sensitive method and that patient age and sperm morphology are associated with centriole quality. Frontiers Media S.A. 2021-04-22 /pmc/articles/PMC8100587/ /pubmed/33968935 http://dx.doi.org/10.3389/fcell.2021.658891 Text en Copyright © 2021 Turner, Fishman, Asadullah, Ott, Dusza, Shah, Sindhwani, Nadiminty, Molinari, Patrizio, Saltzman and Avidor-Reiss. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Turner, Katerina A.
Fishman, Emily L.
Asadullah, Mariam
Ott, Brooke
Dusza, Patrick
Shah, Tariq A.
Sindhwani, Puneet
Nadiminty, Nagalakshmi
Molinari, Emanuela
Patrizio, Pasquale
Saltzman, Barbara S.
Avidor-Reiss, Tomer
Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title_full Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title_fullStr Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title_full_unstemmed Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title_short Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality
title_sort fluorescence-based ratiometric analysis of sperm centrioles (frac) finds patient age and sperm morphology are associated with centriole quality
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100587/
https://www.ncbi.nlm.nih.gov/pubmed/33968935
http://dx.doi.org/10.3389/fcell.2021.658891
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