An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol...

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Detalles Bibliográficos
Autores principales: He, Lingli, Yu, Wentao, Zhang, Wenxiang, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100613/
https://www.ncbi.nlm.nih.gov/pubmed/33997818
http://dx.doi.org/10.1016/j.xpro.2021.100504

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100613/
https://www.ncbi.nlm.nih.gov/pubmed/33997818
http://dx.doi.org/10.1016/j.xpro.2021.100504

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