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An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA
Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100613/ https://www.ncbi.nlm.nih.gov/pubmed/33997818 http://dx.doi.org/10.1016/j.xpro.2021.100504 |