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Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota

BACKGROUND: Low-biomass microbiome studies (such as those of the lungs, placenta, and skin) are vulnerable to contamination and sequencing stochasticity, which obscure legitimate microbial signal. While human lung microbiome studies have rigorously identified sampling strategies that reliably captur...

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Detalles Bibliográficos
Autores principales:	Baker, Jennifer M., Hinkle, Kevin J., McDonald, Roderick A., Brown, Christopher A., Falkowski, Nicole R., Huffnagle, Gary B., Dickson, Robert P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101028/ https://www.ncbi.nlm.nih.gov/pubmed/33952355 http://dx.doi.org/10.1186/s40168-021-01055-4

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