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Osteogenic effects of exosomes derived from human chorion membrane extracts

OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteoge...

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Autores principales: Go, Yoon Young, Chae, Sung-won, Song, Jae-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101178/
https://www.ncbi.nlm.nih.gov/pubmed/33957991
http://dx.doi.org/10.1186/s40824-021-00218-6
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author Go, Yoon Young
Chae, Sung-won
Song, Jae-Jun
author_facet Go, Yoon Young
Chae, Sung-won
Song, Jae-Jun
author_sort Go, Yoon Young
collection PubMed
description OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteogenic effect of CME exosomes on MG63 cells. METHODS: Exosomes were isolated from human placenta CME using the ExoQuick-TC solution and were characterized. The activity and deposition of alkaline phosphatase (ALP) on MG63 cells cultured with or without exosomes in osteogenic induction medium (OIM) were determined. Human amniotic membrane extracts (AME) were used as controls as they had not affected the osteogenic differentiation of MG63 cells in our previous study. RESULTS: Transmission electron microscopy (TEM) revealed that exosomes isolated from CME and AME (CME-Exo and AME-Exo, respectively) had a cup-shaped structure. NanoSight™ particle tracking analysis (NTA) confirmed that the size of these exosomes was 100–150 nm. In vitro osteogenic experiments demonstrated that the exosomes from CME, but not those from AME, presented increased alkaline phosphatase (ALP) activity and resulted in the mineralization of MG63 cells in a dose-dependent manner. CONCLUSION: Exosomes were identified in CME and AME from the human placenta. Further, the exosomes from CME were found to be capable of promoting osteogenic differentiation, suggesting that exosomes are a key component of CME that stimulate the osteogenesis of human osteoblast-like cells. CME exosomes can be developed as promising therapeutic candidates for bone regeneration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-021-00218-6.
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spelling pubmed-81011782021-05-06 Osteogenic effects of exosomes derived from human chorion membrane extracts Go, Yoon Young Chae, Sung-won Song, Jae-Jun Biomater Res Research Article OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteogenic effect of CME exosomes on MG63 cells. METHODS: Exosomes were isolated from human placenta CME using the ExoQuick-TC solution and were characterized. The activity and deposition of alkaline phosphatase (ALP) on MG63 cells cultured with or without exosomes in osteogenic induction medium (OIM) were determined. Human amniotic membrane extracts (AME) were used as controls as they had not affected the osteogenic differentiation of MG63 cells in our previous study. RESULTS: Transmission electron microscopy (TEM) revealed that exosomes isolated from CME and AME (CME-Exo and AME-Exo, respectively) had a cup-shaped structure. NanoSight™ particle tracking analysis (NTA) confirmed that the size of these exosomes was 100–150 nm. In vitro osteogenic experiments demonstrated that the exosomes from CME, but not those from AME, presented increased alkaline phosphatase (ALP) activity and resulted in the mineralization of MG63 cells in a dose-dependent manner. CONCLUSION: Exosomes were identified in CME and AME from the human placenta. Further, the exosomes from CME were found to be capable of promoting osteogenic differentiation, suggesting that exosomes are a key component of CME that stimulate the osteogenesis of human osteoblast-like cells. CME exosomes can be developed as promising therapeutic candidates for bone regeneration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-021-00218-6. BioMed Central 2021-05-06 /pmc/articles/PMC8101178/ /pubmed/33957991 http://dx.doi.org/10.1186/s40824-021-00218-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Go, Yoon Young
Chae, Sung-won
Song, Jae-Jun
Osteogenic effects of exosomes derived from human chorion membrane extracts
title Osteogenic effects of exosomes derived from human chorion membrane extracts
title_full Osteogenic effects of exosomes derived from human chorion membrane extracts
title_fullStr Osteogenic effects of exosomes derived from human chorion membrane extracts
title_full_unstemmed Osteogenic effects of exosomes derived from human chorion membrane extracts
title_short Osteogenic effects of exosomes derived from human chorion membrane extracts
title_sort osteogenic effects of exosomes derived from human chorion membrane extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101178/
https://www.ncbi.nlm.nih.gov/pubmed/33957991
http://dx.doi.org/10.1186/s40824-021-00218-6
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