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Osteogenic effects of exosomes derived from human chorion membrane extracts
OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteoge...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101178/ https://www.ncbi.nlm.nih.gov/pubmed/33957991 http://dx.doi.org/10.1186/s40824-021-00218-6 |
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author | Go, Yoon Young Chae, Sung-won Song, Jae-Jun |
author_facet | Go, Yoon Young Chae, Sung-won Song, Jae-Jun |
author_sort | Go, Yoon Young |
collection | PubMed |
description | OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteogenic effect of CME exosomes on MG63 cells. METHODS: Exosomes were isolated from human placenta CME using the ExoQuick-TC solution and were characterized. The activity and deposition of alkaline phosphatase (ALP) on MG63 cells cultured with or without exosomes in osteogenic induction medium (OIM) were determined. Human amniotic membrane extracts (AME) were used as controls as they had not affected the osteogenic differentiation of MG63 cells in our previous study. RESULTS: Transmission electron microscopy (TEM) revealed that exosomes isolated from CME and AME (CME-Exo and AME-Exo, respectively) had a cup-shaped structure. NanoSight™ particle tracking analysis (NTA) confirmed that the size of these exosomes was 100–150 nm. In vitro osteogenic experiments demonstrated that the exosomes from CME, but not those from AME, presented increased alkaline phosphatase (ALP) activity and resulted in the mineralization of MG63 cells in a dose-dependent manner. CONCLUSION: Exosomes were identified in CME and AME from the human placenta. Further, the exosomes from CME were found to be capable of promoting osteogenic differentiation, suggesting that exosomes are a key component of CME that stimulate the osteogenesis of human osteoblast-like cells. CME exosomes can be developed as promising therapeutic candidates for bone regeneration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-021-00218-6. |
format | Online Article Text |
id | pubmed-8101178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81011782021-05-06 Osteogenic effects of exosomes derived from human chorion membrane extracts Go, Yoon Young Chae, Sung-won Song, Jae-Jun Biomater Res Research Article OBJECTIVE: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteogenic effect of CME exosomes on MG63 cells. METHODS: Exosomes were isolated from human placenta CME using the ExoQuick-TC solution and were characterized. The activity and deposition of alkaline phosphatase (ALP) on MG63 cells cultured with or without exosomes in osteogenic induction medium (OIM) were determined. Human amniotic membrane extracts (AME) were used as controls as they had not affected the osteogenic differentiation of MG63 cells in our previous study. RESULTS: Transmission electron microscopy (TEM) revealed that exosomes isolated from CME and AME (CME-Exo and AME-Exo, respectively) had a cup-shaped structure. NanoSight™ particle tracking analysis (NTA) confirmed that the size of these exosomes was 100–150 nm. In vitro osteogenic experiments demonstrated that the exosomes from CME, but not those from AME, presented increased alkaline phosphatase (ALP) activity and resulted in the mineralization of MG63 cells in a dose-dependent manner. CONCLUSION: Exosomes were identified in CME and AME from the human placenta. Further, the exosomes from CME were found to be capable of promoting osteogenic differentiation, suggesting that exosomes are a key component of CME that stimulate the osteogenesis of human osteoblast-like cells. CME exosomes can be developed as promising therapeutic candidates for bone regeneration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-021-00218-6. BioMed Central 2021-05-06 /pmc/articles/PMC8101178/ /pubmed/33957991 http://dx.doi.org/10.1186/s40824-021-00218-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Go, Yoon Young Chae, Sung-won Song, Jae-Jun Osteogenic effects of exosomes derived from human chorion membrane extracts |
title | Osteogenic effects of exosomes derived from human chorion membrane extracts |
title_full | Osteogenic effects of exosomes derived from human chorion membrane extracts |
title_fullStr | Osteogenic effects of exosomes derived from human chorion membrane extracts |
title_full_unstemmed | Osteogenic effects of exosomes derived from human chorion membrane extracts |
title_short | Osteogenic effects of exosomes derived from human chorion membrane extracts |
title_sort | osteogenic effects of exosomes derived from human chorion membrane extracts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101178/ https://www.ncbi.nlm.nih.gov/pubmed/33957991 http://dx.doi.org/10.1186/s40824-021-00218-6 |
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