Cargando…

Coverage and quality of DNA barcode references for Central and Northern European Odonata

BACKGROUND: Dragonflies and damselflies (Odonata) are important components in biomonitoring due to their amphibiotic lifecycle and specific habitat requirements. They are charismatic and popular insects, but can be challenging to identify despite large size and often distinct coloration, especially...

Descripción completa

Detalles Bibliográficos
Autores principales: Geiger, Matthias, Koblmüller, Stephan, Assandri, Giacomo, Chovanec, Andreas, Ekrem, Torbjørn, Fischer, Iris, Galimberti, Andrea, Grabowski, Michał, Haring, Elisabeth, Hausmann, Axel, Hendrich, Lars, Koch, Stefan, Mamos, Tomasz, Rothe, Udo, Rulik, Björn, Rewicz, Tomasz, Sittenthaler, Marcia, Stur, Elisabeth, Tończyk, Grzegorz, Zangl, Lukas, Moriniere, Jerome
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101477/
https://www.ncbi.nlm.nih.gov/pubmed/33986985
http://dx.doi.org/10.7717/peerj.11192
_version_ 1783688959583322112
author Geiger, Matthias
Koblmüller, Stephan
Assandri, Giacomo
Chovanec, Andreas
Ekrem, Torbjørn
Fischer, Iris
Galimberti, Andrea
Grabowski, Michał
Haring, Elisabeth
Hausmann, Axel
Hendrich, Lars
Koch, Stefan
Mamos, Tomasz
Rothe, Udo
Rulik, Björn
Rewicz, Tomasz
Sittenthaler, Marcia
Stur, Elisabeth
Tończyk, Grzegorz
Zangl, Lukas
Moriniere, Jerome
author_facet Geiger, Matthias
Koblmüller, Stephan
Assandri, Giacomo
Chovanec, Andreas
Ekrem, Torbjørn
Fischer, Iris
Galimberti, Andrea
Grabowski, Michał
Haring, Elisabeth
Hausmann, Axel
Hendrich, Lars
Koch, Stefan
Mamos, Tomasz
Rothe, Udo
Rulik, Björn
Rewicz, Tomasz
Sittenthaler, Marcia
Stur, Elisabeth
Tończyk, Grzegorz
Zangl, Lukas
Moriniere, Jerome
author_sort Geiger, Matthias
collection PubMed
description BACKGROUND: Dragonflies and damselflies (Odonata) are important components in biomonitoring due to their amphibiotic lifecycle and specific habitat requirements. They are charismatic and popular insects, but can be challenging to identify despite large size and often distinct coloration, especially the immature stages. DNA-based assessment tools rely on validated DNA barcode reference libraries evaluated in a supraregional context to minimize taxonomic incongruence and identification mismatches. METHODS: This study reports on findings from the analysis of the most comprehensive DNA barcode dataset for Central European Odonata to date, with 103 out of 145 recorded European species included and publicly deposited in the Barcode of Life Data System (BOLD). The complete dataset includes 697 specimens (548 adults, 108 larvae) from 274 localities in 16 countries with a geographic emphasis on Central Europe. We used BOLD to generate sequence divergence metrics and to examine the taxonomic composition of the DNA barcode clusters within the dataset and in comparison with all data on BOLD. RESULTS: Over 88% of the species included can be readily identified using their DNA barcodes and the reference dataset provided. Considering the complete European dataset, unambiguous identification is hampered in 12 species due to weak mitochondrial differentiation and partial haplotype sharing. However, considering the known species distributions only two groups of five species possibly co-occur, leading to an unambiguous identification of more than 95% of the analysed Odonata via DNA barcoding in real applications. The cases of small interspecific genetic distances and the observed deep intraspecific variation in Cordulia aenea (Linnaeus, 1758) are discussed in detail and the corresponding taxa in the public reference database are highlighted. They should be considered in future applications of DNA barcoding and metabarcoding and represent interesting evolutionary biological questions, which call for in depth analyses of the involved taxa throughout their distribution ranges.
format Online
Article
Text
id pubmed-8101477
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-81014772021-05-12 Coverage and quality of DNA barcode references for Central and Northern European Odonata Geiger, Matthias Koblmüller, Stephan Assandri, Giacomo Chovanec, Andreas Ekrem, Torbjørn Fischer, Iris Galimberti, Andrea Grabowski, Michał Haring, Elisabeth Hausmann, Axel Hendrich, Lars Koch, Stefan Mamos, Tomasz Rothe, Udo Rulik, Björn Rewicz, Tomasz Sittenthaler, Marcia Stur, Elisabeth Tończyk, Grzegorz Zangl, Lukas Moriniere, Jerome PeerJ Entomology BACKGROUND: Dragonflies and damselflies (Odonata) are important components in biomonitoring due to their amphibiotic lifecycle and specific habitat requirements. They are charismatic and popular insects, but can be challenging to identify despite large size and often distinct coloration, especially the immature stages. DNA-based assessment tools rely on validated DNA barcode reference libraries evaluated in a supraregional context to minimize taxonomic incongruence and identification mismatches. METHODS: This study reports on findings from the analysis of the most comprehensive DNA barcode dataset for Central European Odonata to date, with 103 out of 145 recorded European species included and publicly deposited in the Barcode of Life Data System (BOLD). The complete dataset includes 697 specimens (548 adults, 108 larvae) from 274 localities in 16 countries with a geographic emphasis on Central Europe. We used BOLD to generate sequence divergence metrics and to examine the taxonomic composition of the DNA barcode clusters within the dataset and in comparison with all data on BOLD. RESULTS: Over 88% of the species included can be readily identified using their DNA barcodes and the reference dataset provided. Considering the complete European dataset, unambiguous identification is hampered in 12 species due to weak mitochondrial differentiation and partial haplotype sharing. However, considering the known species distributions only two groups of five species possibly co-occur, leading to an unambiguous identification of more than 95% of the analysed Odonata via DNA barcoding in real applications. The cases of small interspecific genetic distances and the observed deep intraspecific variation in Cordulia aenea (Linnaeus, 1758) are discussed in detail and the corresponding taxa in the public reference database are highlighted. They should be considered in future applications of DNA barcoding and metabarcoding and represent interesting evolutionary biological questions, which call for in depth analyses of the involved taxa throughout their distribution ranges. PeerJ Inc. 2021-05-03 /pmc/articles/PMC8101477/ /pubmed/33986985 http://dx.doi.org/10.7717/peerj.11192 Text en © 2021 Geiger et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Entomology
Geiger, Matthias
Koblmüller, Stephan
Assandri, Giacomo
Chovanec, Andreas
Ekrem, Torbjørn
Fischer, Iris
Galimberti, Andrea
Grabowski, Michał
Haring, Elisabeth
Hausmann, Axel
Hendrich, Lars
Koch, Stefan
Mamos, Tomasz
Rothe, Udo
Rulik, Björn
Rewicz, Tomasz
Sittenthaler, Marcia
Stur, Elisabeth
Tończyk, Grzegorz
Zangl, Lukas
Moriniere, Jerome
Coverage and quality of DNA barcode references for Central and Northern European Odonata
title Coverage and quality of DNA barcode references for Central and Northern European Odonata
title_full Coverage and quality of DNA barcode references for Central and Northern European Odonata
title_fullStr Coverage and quality of DNA barcode references for Central and Northern European Odonata
title_full_unstemmed Coverage and quality of DNA barcode references for Central and Northern European Odonata
title_short Coverage and quality of DNA barcode references for Central and Northern European Odonata
title_sort coverage and quality of dna barcode references for central and northern european odonata
topic Entomology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101477/
https://www.ncbi.nlm.nih.gov/pubmed/33986985
http://dx.doi.org/10.7717/peerj.11192
work_keys_str_mv AT geigermatthias coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT koblmullerstephan coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT assandrigiacomo coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT chovanecandreas coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT ekremtorbjørn coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT fischeriris coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT galimbertiandrea coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT grabowskimichał coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT haringelisabeth coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT hausmannaxel coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT hendrichlars coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT kochstefan coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT mamostomasz coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT rotheudo coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT rulikbjorn coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT rewicztomasz coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT sittenthalermarcia coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT sturelisabeth coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT tonczykgrzegorz coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT zangllukas coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata
AT morinierejerome coverageandqualityofdnabarcodereferencesforcentralandnortherneuropeanodonata