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Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China

BACKGROUND: Mosquitoes host and transmit numerous arthropod-borne viruses (arboviruses) that cause disease in both humans and animals. Effective surveillance of virome profiles in mosquitoes is vital to the prevention and control of mosquito-borne diseases in northwestern China, where epidemics occu...

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Autores principales: He, Xiaozhou, Yin, Qikai, Zhou, Liwei, Meng, Lei, Hu, Weijun, Li, Fan, Li, Yang, Han, Kun, Zhang, Shaobai, Fu, Shihong, Zhang, Xiaoshu, Wang, Ji, Xu, Songtao, Zhang, Yi, He, Ying, Dong, Maoxing, Shen, Xinxin, Zhang, Zheng, Nie, Kai, Liang, Guodong, Ma, Xuejun, Wang, Huanyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101993/
https://www.ncbi.nlm.nih.gov/pubmed/33901182
http://dx.doi.org/10.1371/journal.pntd.0009381
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author He, Xiaozhou
Yin, Qikai
Zhou, Liwei
Meng, Lei
Hu, Weijun
Li, Fan
Li, Yang
Han, Kun
Zhang, Shaobai
Fu, Shihong
Zhang, Xiaoshu
Wang, Ji
Xu, Songtao
Zhang, Yi
He, Ying
Dong, Maoxing
Shen, Xinxin
Zhang, Zheng
Nie, Kai
Liang, Guodong
Ma, Xuejun
Wang, Huanyu
author_facet He, Xiaozhou
Yin, Qikai
Zhou, Liwei
Meng, Lei
Hu, Weijun
Li, Fan
Li, Yang
Han, Kun
Zhang, Shaobai
Fu, Shihong
Zhang, Xiaoshu
Wang, Ji
Xu, Songtao
Zhang, Yi
He, Ying
Dong, Maoxing
Shen, Xinxin
Zhang, Zheng
Nie, Kai
Liang, Guodong
Ma, Xuejun
Wang, Huanyu
author_sort He, Xiaozhou
collection PubMed
description BACKGROUND: Mosquitoes host and transmit numerous arthropod-borne viruses (arboviruses) that cause disease in both humans and animals. Effective surveillance of virome profiles in mosquitoes is vital to the prevention and control of mosquito-borne diseases in northwestern China, where epidemics occur frequently. METHODS: Mosquitoes were collected in the Shaanxi-Gansu-Ningxia region (Shaanxi Province, Gansu Province, and Ningxia Hui Autonomous Region) of China from June to August 2019. Morphological methods were used for taxonomic identification of mosquito species. High-throughput sequencing and metagenomic analysis were used to characterize mosquito viromes. RESULTS: A total of 22,959 mosquitoes were collected, including Culex pipiens (45.7%), Culex tritaeniorhynchus (40.6%), Anopheles sinensis (8.4%), Aedes (5.2%), and Armigeres subalbatus (0.1%). In total, 3,014,183 (0.95% of clean reads) viral sequences were identified and assigned to 116 viral species (including pathogens such as Japanese encephalitis virus and Getah virus) in 31 viral families, including Flaviviridae, Togaviridae, Phasmaviridae, Phenuiviridae, and some unclassified viruses. Mosquitoes collected in July (86 species in 26 families) showed greater viral diversity than those from June and August. Culex pipiens (69 species in 25 families) and Culex tritaeniorhynchus (73 species in 24 families) carried more viral species than Anopheles sinensis (50 species in 19 families) or Aedes (38 species in 20 families) mosquitoes. CONCLUSION: Viral diversity and abundance were affected by mosquito species and collection time. The present study elucidates the virome compositions of various mosquito species in northwestern China, improving the understanding of virus transmission dynamics for comparison with those of disease outbreaks.
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spelling pubmed-81019932021-05-17 Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China He, Xiaozhou Yin, Qikai Zhou, Liwei Meng, Lei Hu, Weijun Li, Fan Li, Yang Han, Kun Zhang, Shaobai Fu, Shihong Zhang, Xiaoshu Wang, Ji Xu, Songtao Zhang, Yi He, Ying Dong, Maoxing Shen, Xinxin Zhang, Zheng Nie, Kai Liang, Guodong Ma, Xuejun Wang, Huanyu PLoS Negl Trop Dis Research Article BACKGROUND: Mosquitoes host and transmit numerous arthropod-borne viruses (arboviruses) that cause disease in both humans and animals. Effective surveillance of virome profiles in mosquitoes is vital to the prevention and control of mosquito-borne diseases in northwestern China, where epidemics occur frequently. METHODS: Mosquitoes were collected in the Shaanxi-Gansu-Ningxia region (Shaanxi Province, Gansu Province, and Ningxia Hui Autonomous Region) of China from June to August 2019. Morphological methods were used for taxonomic identification of mosquito species. High-throughput sequencing and metagenomic analysis were used to characterize mosquito viromes. RESULTS: A total of 22,959 mosquitoes were collected, including Culex pipiens (45.7%), Culex tritaeniorhynchus (40.6%), Anopheles sinensis (8.4%), Aedes (5.2%), and Armigeres subalbatus (0.1%). In total, 3,014,183 (0.95% of clean reads) viral sequences were identified and assigned to 116 viral species (including pathogens such as Japanese encephalitis virus and Getah virus) in 31 viral families, including Flaviviridae, Togaviridae, Phasmaviridae, Phenuiviridae, and some unclassified viruses. Mosquitoes collected in July (86 species in 26 families) showed greater viral diversity than those from June and August. Culex pipiens (69 species in 25 families) and Culex tritaeniorhynchus (73 species in 24 families) carried more viral species than Anopheles sinensis (50 species in 19 families) or Aedes (38 species in 20 families) mosquitoes. CONCLUSION: Viral diversity and abundance were affected by mosquito species and collection time. The present study elucidates the virome compositions of various mosquito species in northwestern China, improving the understanding of virus transmission dynamics for comparison with those of disease outbreaks. Public Library of Science 2021-04-26 /pmc/articles/PMC8101993/ /pubmed/33901182 http://dx.doi.org/10.1371/journal.pntd.0009381 Text en © 2021 He et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
He, Xiaozhou
Yin, Qikai
Zhou, Liwei
Meng, Lei
Hu, Weijun
Li, Fan
Li, Yang
Han, Kun
Zhang, Shaobai
Fu, Shihong
Zhang, Xiaoshu
Wang, Ji
Xu, Songtao
Zhang, Yi
He, Ying
Dong, Maoxing
Shen, Xinxin
Zhang, Zheng
Nie, Kai
Liang, Guodong
Ma, Xuejun
Wang, Huanyu
Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title_full Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title_fullStr Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title_full_unstemmed Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title_short Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China
title_sort metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the shaanxi-gansu-ningxia region, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101993/
https://www.ncbi.nlm.nih.gov/pubmed/33901182
http://dx.doi.org/10.1371/journal.pntd.0009381
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