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Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation
The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102097/ https://www.ncbi.nlm.nih.gov/pubmed/34007285 http://dx.doi.org/10.1155/2021/5595172 |
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author | Wolmarans, Elize Mellet, Juanita Durandt, Chrisna Joubert, Fourie Pepper, Michael S. |
author_facet | Wolmarans, Elize Mellet, Juanita Durandt, Chrisna Joubert, Fourie Pepper, Michael S. |
author_sort | Wolmarans, Elize |
collection | PubMed |
description | The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs). |
format | Online Article Text |
id | pubmed-8102097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-81020972021-05-17 Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation Wolmarans, Elize Mellet, Juanita Durandt, Chrisna Joubert, Fourie Pepper, Michael S. Stem Cells Int Research Article The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs). Hindawi 2021-04-28 /pmc/articles/PMC8102097/ /pubmed/34007285 http://dx.doi.org/10.1155/2021/5595172 Text en Copyright © 2021 Elize Wolmarans et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wolmarans, Elize Mellet, Juanita Durandt, Chrisna Joubert, Fourie Pepper, Michael S. Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title | Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title_full | Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title_fullStr | Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title_full_unstemmed | Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title_short | Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation |
title_sort | single-cell transcriptome analysis of human adipose-derived stromal cells identifies a contractile cell subpopulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102097/ https://www.ncbi.nlm.nih.gov/pubmed/34007285 http://dx.doi.org/10.1155/2021/5595172 |
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