Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH

Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH us...

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Autores principales: Daemen, Sabine, Chan, Mandy M., Schilling, Joel D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102804/
https://www.ncbi.nlm.nih.gov/pubmed/33997821
http://dx.doi.org/10.1016/j.xpro.2021.100511
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author Daemen, Sabine
Chan, Mandy M.
Schilling, Joel D.
author_facet Daemen, Sabine
Chan, Mandy M.
Schilling, Joel D.
author_sort Daemen, Sabine
collection PubMed
description Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).
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spelling pubmed-81028042021-05-14 Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH Daemen, Sabine Chan, Mandy M. Schilling, Joel D. STAR Protoc Protocol Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021). Elsevier 2021-04-29 /pmc/articles/PMC8102804/ /pubmed/33997821 http://dx.doi.org/10.1016/j.xpro.2021.100511 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Daemen, Sabine
Chan, Mandy M.
Schilling, Joel D.
Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title_full Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title_fullStr Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title_full_unstemmed Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title_short Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
title_sort comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine nash
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102804/
https://www.ncbi.nlm.nih.gov/pubmed/33997821
http://dx.doi.org/10.1016/j.xpro.2021.100511
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