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Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.

Centipedegrass [Eremochloa ophiuroides (Munro) Hack.] is a perennial warm-season grass that originated in China, and its speed of nodal rooting is important for lawn establishment. In our study, centipedegrass nodal rooting ability was limited by node aging. Transcriptome sequencing of nodal roots a...

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Autores principales: Wang, Rui, Zhao, Haoyan, Guo, Hailin, Zong, Junqin, Li, Jianjian, Wang, Haoran, Liu, Jianxiu, Wang, Jingjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102984/
https://www.ncbi.nlm.nih.gov/pubmed/33968116
http://dx.doi.org/10.3389/fpls.2021.659830
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author Wang, Rui
Zhao, Haoyan
Guo, Hailin
Zong, Junqin
Li, Jianjian
Wang, Haoran
Liu, Jianxiu
Wang, Jingjing
author_facet Wang, Rui
Zhao, Haoyan
Guo, Hailin
Zong, Junqin
Li, Jianjian
Wang, Haoran
Liu, Jianxiu
Wang, Jingjing
author_sort Wang, Rui
collection PubMed
description Centipedegrass [Eremochloa ophiuroides (Munro) Hack.] is a perennial warm-season grass that originated in China, and its speed of nodal rooting is important for lawn establishment. In our study, centipedegrass nodal rooting ability was limited by node aging. Transcriptome sequencing of nodal roots after 0, 2, 4, and 8 days of water culture was performed to investigate the molecular mechanisms of root development. GO enrichment and KEGG pathway analyses of DEGs indicated that plant hormone signal transduction and transcription factors might play important roles in centipedegrass nodal root growth. Among them, E3 ubiquitin-protein ligases participated in multiple hormone signal transduction pathways and interacted with transcription factors. Furthermore, an E3 ubiquitin protein ligase EoSINAT5 overexpressed in rice resulted in longer roots and more numerous root tips, while knockout of LOC_Os07g46560 (the homologous gene of EoSINAT5 in rice) resulted in shorter roots and fewer root tips. These results indicated that EoSINAT5 and its homologous gene are able to promote nodal root development. This research presents the transcriptomic analyses of centipedegrass nodal roots, and may contribute to elucidating the mechanism governing the development of nodal roots and facilitates the use of molecular breeding in improving rooting ability.
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spelling pubmed-81029842021-05-08 Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack. Wang, Rui Zhao, Haoyan Guo, Hailin Zong, Junqin Li, Jianjian Wang, Haoran Liu, Jianxiu Wang, Jingjing Front Plant Sci Plant Science Centipedegrass [Eremochloa ophiuroides (Munro) Hack.] is a perennial warm-season grass that originated in China, and its speed of nodal rooting is important for lawn establishment. In our study, centipedegrass nodal rooting ability was limited by node aging. Transcriptome sequencing of nodal roots after 0, 2, 4, and 8 days of water culture was performed to investigate the molecular mechanisms of root development. GO enrichment and KEGG pathway analyses of DEGs indicated that plant hormone signal transduction and transcription factors might play important roles in centipedegrass nodal root growth. Among them, E3 ubiquitin-protein ligases participated in multiple hormone signal transduction pathways and interacted with transcription factors. Furthermore, an E3 ubiquitin protein ligase EoSINAT5 overexpressed in rice resulted in longer roots and more numerous root tips, while knockout of LOC_Os07g46560 (the homologous gene of EoSINAT5 in rice) resulted in shorter roots and fewer root tips. These results indicated that EoSINAT5 and its homologous gene are able to promote nodal root development. This research presents the transcriptomic analyses of centipedegrass nodal roots, and may contribute to elucidating the mechanism governing the development of nodal roots and facilitates the use of molecular breeding in improving rooting ability. Frontiers Media S.A. 2021-04-23 /pmc/articles/PMC8102984/ /pubmed/33968116 http://dx.doi.org/10.3389/fpls.2021.659830 Text en Copyright © 2021 Wang, Zhao, Guo, Zong, Li, Wang, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Rui
Zhao, Haoyan
Guo, Hailin
Zong, Junqin
Li, Jianjian
Wang, Haoran
Liu, Jianxiu
Wang, Jingjing
Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title_full Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title_fullStr Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title_full_unstemmed Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title_short Use of Transcriptomic Analyses to Elucidate the Mechanism Governing Nodal Root Development in Eremochloa ophiuroides (Munro) Hack.
title_sort use of transcriptomic analyses to elucidate the mechanism governing nodal root development in eremochloa ophiuroides (munro) hack.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102984/
https://www.ncbi.nlm.nih.gov/pubmed/33968116
http://dx.doi.org/10.3389/fpls.2021.659830
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