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Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders

Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screen...

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Autores principales: Peng, Ye, Chu, Shifeng, Yang, Yantao, Zhang, Zhao, Pang, Zongran, Chen, Naihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103160/
https://www.ncbi.nlm.nih.gov/pubmed/33967814
http://dx.doi.org/10.3389/fphar.2021.671734
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author Peng, Ye
Chu, Shifeng
Yang, Yantao
Zhang, Zhao
Pang, Zongran
Chen, Naihong
author_facet Peng, Ye
Chu, Shifeng
Yang, Yantao
Zhang, Zhao
Pang, Zongran
Chen, Naihong
author_sort Peng, Ye
collection PubMed
description Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screening is urgent. Neuronal and glial cell models have been widely used in the researches of neurological disorders. And the current researches on neuroinflammation contributes to blood-brain barrier (BBB) damage. In this review, we describe the features of healthy and inflamed BBB and summarize the main immortalized cell lines of the central nervous system (PC12, SH-SY5Y, BV2, HA, and HBMEC et al.) and their use in the anti-inflammatory potential of neurological disorders. Especially, different co-culture models of neuroinflammatory, in association with immune cells in both 2D and 3D models are discussed in this review. In summary, 2D co-culture is easily practicable and economical but cannot fully reproduce the microenvironment in vivo. While 3D models called organs-on-chips or biochips are the most recent and very promising approach, which made possible by bioengineering and biotechnological improvements and more accurately mimic the BBB microenvironment.
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spelling pubmed-81031602021-05-08 Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders Peng, Ye Chu, Shifeng Yang, Yantao Zhang, Zhao Pang, Zongran Chen, Naihong Front Pharmacol Pharmacology Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screening is urgent. Neuronal and glial cell models have been widely used in the researches of neurological disorders. And the current researches on neuroinflammation contributes to blood-brain barrier (BBB) damage. In this review, we describe the features of healthy and inflamed BBB and summarize the main immortalized cell lines of the central nervous system (PC12, SH-SY5Y, BV2, HA, and HBMEC et al.) and their use in the anti-inflammatory potential of neurological disorders. Especially, different co-culture models of neuroinflammatory, in association with immune cells in both 2D and 3D models are discussed in this review. In summary, 2D co-culture is easily practicable and economical but cannot fully reproduce the microenvironment in vivo. While 3D models called organs-on-chips or biochips are the most recent and very promising approach, which made possible by bioengineering and biotechnological improvements and more accurately mimic the BBB microenvironment. Frontiers Media S.A. 2021-04-23 /pmc/articles/PMC8103160/ /pubmed/33967814 http://dx.doi.org/10.3389/fphar.2021.671734 Text en Copyright © 2021 Peng, Chu, Yang, Zhang, Pang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Peng, Ye
Chu, Shifeng
Yang, Yantao
Zhang, Zhao
Pang, Zongran
Chen, Naihong
Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title_full Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title_fullStr Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title_full_unstemmed Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title_short Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
title_sort neuroinflammatory in vitro cell culture models and the potential applications for neurological disorders
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103160/
https://www.ncbi.nlm.nih.gov/pubmed/33967814
http://dx.doi.org/10.3389/fphar.2021.671734
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