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Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine

A 2(3) full factorial design model was used for the development of a new high performance liquid chromatography method with UV detection to estimate three antifungal drugs simultaneously. Fluconazole (FLU), itraconazole (ITR) and terbinafine (TRH) are co-administered for severe fungal infections. Th...

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Autores principales: Roshdy, Aya, Elmansi, Heba, Shalan, Shereen, El-Brashy, Amina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103232/
https://www.ncbi.nlm.nih.gov/pubmed/33972882
http://dx.doi.org/10.1098/rsos.202130
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author Roshdy, Aya
Elmansi, Heba
Shalan, Shereen
El-Brashy, Amina
author_facet Roshdy, Aya
Elmansi, Heba
Shalan, Shereen
El-Brashy, Amina
author_sort Roshdy, Aya
collection PubMed
description A 2(3) full factorial design model was used for the development of a new high performance liquid chromatography method with UV detection to estimate three antifungal drugs simultaneously. Fluconazole (FLU), itraconazole (ITR) and terbinafine (TRH) are co-administered for severe fungal infections. They have been determined using MOS-1 Hypersil C(18) column and an isocratic eluent; methanol 95% and phosphate buffer 5% with 0.001% triethylamine. The pH was adjusted to 7, and the flow rate was 0.7 ml min(−1). The three drugs were separated within less than 7 min at 210 nm. The developed method gave a linear response over 5–80 µg ml(−1), 5–50 µg ml(−1) and 1–50 µg ml(−1) for FLU, ITR and TRH, respectively. It showed detection limits of 0.88, 0.29 and 0.20 µg ml(−1) and quantification limits of 2.66, 0.88 and 0.60 µg ml(−1) for the three drugs, respectively. The design of the experiment facilitated the optimization of different variables affecting the separation of the three drugs. The sensitivity of the designed method permitted the simultaneous estimation of ITR and TRH in spiked human plasma successfully.
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spelling pubmed-81032322021-05-09 Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine Roshdy, Aya Elmansi, Heba Shalan, Shereen El-Brashy, Amina R Soc Open Sci Chemistry A 2(3) full factorial design model was used for the development of a new high performance liquid chromatography method with UV detection to estimate three antifungal drugs simultaneously. Fluconazole (FLU), itraconazole (ITR) and terbinafine (TRH) are co-administered for severe fungal infections. They have been determined using MOS-1 Hypersil C(18) column and an isocratic eluent; methanol 95% and phosphate buffer 5% with 0.001% triethylamine. The pH was adjusted to 7, and the flow rate was 0.7 ml min(−1). The three drugs were separated within less than 7 min at 210 nm. The developed method gave a linear response over 5–80 µg ml(−1), 5–50 µg ml(−1) and 1–50 µg ml(−1) for FLU, ITR and TRH, respectively. It showed detection limits of 0.88, 0.29 and 0.20 µg ml(−1) and quantification limits of 2.66, 0.88 and 0.60 µg ml(−1) for the three drugs, respectively. The design of the experiment facilitated the optimization of different variables affecting the separation of the three drugs. The sensitivity of the designed method permitted the simultaneous estimation of ITR and TRH in spiked human plasma successfully. The Royal Society 2021-02-17 /pmc/articles/PMC8103232/ /pubmed/33972882 http://dx.doi.org/10.1098/rsos.202130 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Roshdy, Aya
Elmansi, Heba
Shalan, Shereen
El-Brashy, Amina
Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title_full Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title_fullStr Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title_full_unstemmed Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title_short Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
title_sort factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103232/
https://www.ncbi.nlm.nih.gov/pubmed/33972882
http://dx.doi.org/10.1098/rsos.202130
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