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Three founding ancestral genomes involved in the origin of sugarcane
BACKGROUND AND AIMS: Modern sugarcane cultivars (Saccharum spp.) are high polyploids, aneuploids (2n = ~12x = ~120) derived from interspecific hybridizations between the domesticated sweet species Saccharum officinarum and the wild species S. spontaneum. METHODS: To analyse the architecture and orig...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103802/ https://www.ncbi.nlm.nih.gov/pubmed/33637991 http://dx.doi.org/10.1093/aob/mcab008 |
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author | Pompidor, Nicolas Charron, Carine Hervouet, Catherine Bocs, Stéphanie Droc, Gaëtan Rivallan, Ronan Manez, Aurore Mitros, Therese Swaminathan, Kankshita Glaszmann, Jean-Christophe Garsmeur, Olivier D’Hont, Angélique |
author_facet | Pompidor, Nicolas Charron, Carine Hervouet, Catherine Bocs, Stéphanie Droc, Gaëtan Rivallan, Ronan Manez, Aurore Mitros, Therese Swaminathan, Kankshita Glaszmann, Jean-Christophe Garsmeur, Olivier D’Hont, Angélique |
author_sort | Pompidor, Nicolas |
collection | PubMed |
description | BACKGROUND AND AIMS: Modern sugarcane cultivars (Saccharum spp.) are high polyploids, aneuploids (2n = ~12x = ~120) derived from interspecific hybridizations between the domesticated sweet species Saccharum officinarum and the wild species S. spontaneum. METHODS: To analyse the architecture and origin of such a complex genome, we analysed the sequences of all 12 hom(oe)ologous haplotypes (BAC clones) from two distinct genomic regions of a typical modern cultivar, as well as the corresponding sequence in Miscanthus sinense and Sorghum bicolor, and monitored their distribution among representatives of the Saccharum genus. KEY RESULTS: The diversity observed among haplotypes suggested the existence of three founding genomes (A, B, C) in modern cultivars, which diverged between 0.8 and 1.3 Mya. Two genomes (A, B) were contributed by S. officinarum; these were also found in its wild presumed ancestor S. robustum, and one genome (C) was contributed by S. spontaneum. These results suggest that S. officinarum and S. robustum are derived from interspecific hybridization between two unknown ancestors (A and B genomes). The A genome contributed most haplotypes (nine or ten) while the B and C genomes contributed one or two haplotypes in the regions analysed of this typical modern cultivar. Interspecific hybridizations likely involved accessions or gametes with distinct ploidy levels and/or were followed by a series of backcrosses with the A genome. The three founding genomes were found in all S. barberi, S. sinense and modern cultivars analysed. None of the analysed accessions contained only the A genome or the B genome, suggesting that representatives of these founding genomes remain to be discovered. CONCLUSIONS: This evolutionary model, which combines interspecificity and high polyploidy, can explain the variable chromosome pairing affinity observed in Saccharum. It represents a major revision of the understanding of Saccharum diversity. |
format | Online Article Text |
id | pubmed-8103802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81038022021-05-11 Three founding ancestral genomes involved in the origin of sugarcane Pompidor, Nicolas Charron, Carine Hervouet, Catherine Bocs, Stéphanie Droc, Gaëtan Rivallan, Ronan Manez, Aurore Mitros, Therese Swaminathan, Kankshita Glaszmann, Jean-Christophe Garsmeur, Olivier D’Hont, Angélique Ann Bot Original Articles BACKGROUND AND AIMS: Modern sugarcane cultivars (Saccharum spp.) are high polyploids, aneuploids (2n = ~12x = ~120) derived from interspecific hybridizations between the domesticated sweet species Saccharum officinarum and the wild species S. spontaneum. METHODS: To analyse the architecture and origin of such a complex genome, we analysed the sequences of all 12 hom(oe)ologous haplotypes (BAC clones) from two distinct genomic regions of a typical modern cultivar, as well as the corresponding sequence in Miscanthus sinense and Sorghum bicolor, and monitored their distribution among representatives of the Saccharum genus. KEY RESULTS: The diversity observed among haplotypes suggested the existence of three founding genomes (A, B, C) in modern cultivars, which diverged between 0.8 and 1.3 Mya. Two genomes (A, B) were contributed by S. officinarum; these were also found in its wild presumed ancestor S. robustum, and one genome (C) was contributed by S. spontaneum. These results suggest that S. officinarum and S. robustum are derived from interspecific hybridization between two unknown ancestors (A and B genomes). The A genome contributed most haplotypes (nine or ten) while the B and C genomes contributed one or two haplotypes in the regions analysed of this typical modern cultivar. Interspecific hybridizations likely involved accessions or gametes with distinct ploidy levels and/or were followed by a series of backcrosses with the A genome. The three founding genomes were found in all S. barberi, S. sinense and modern cultivars analysed. None of the analysed accessions contained only the A genome or the B genome, suggesting that representatives of these founding genomes remain to be discovered. CONCLUSIONS: This evolutionary model, which combines interspecificity and high polyploidy, can explain the variable chromosome pairing affinity observed in Saccharum. It represents a major revision of the understanding of Saccharum diversity. Oxford University Press 2021-02-26 /pmc/articles/PMC8103802/ /pubmed/33637991 http://dx.doi.org/10.1093/aob/mcab008 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Pompidor, Nicolas Charron, Carine Hervouet, Catherine Bocs, Stéphanie Droc, Gaëtan Rivallan, Ronan Manez, Aurore Mitros, Therese Swaminathan, Kankshita Glaszmann, Jean-Christophe Garsmeur, Olivier D’Hont, Angélique Three founding ancestral genomes involved in the origin of sugarcane |
title | Three founding ancestral genomes involved in the origin of sugarcane |
title_full | Three founding ancestral genomes involved in the origin of sugarcane |
title_fullStr | Three founding ancestral genomes involved in the origin of sugarcane |
title_full_unstemmed | Three founding ancestral genomes involved in the origin of sugarcane |
title_short | Three founding ancestral genomes involved in the origin of sugarcane |
title_sort | three founding ancestral genomes involved in the origin of sugarcane |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103802/ https://www.ncbi.nlm.nih.gov/pubmed/33637991 http://dx.doi.org/10.1093/aob/mcab008 |
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