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Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction

Eukaryotic and prokaryotic cell membranes are difficult to characterize directly with biophysical methods. Membrane model systems, that include fewer molecular species, are therefore often used to reproduce their fundamental chemical and physical properties. In this context, natural lipid mixtures d...

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Autores principales: Luchini, Alessandra, Corucci, Giacomo, Chaithanya Batchu, Krishna, Laux, Valerie, Haertlein, Michael, Cristiglio, Viviana, Fragneto, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104085/
https://www.ncbi.nlm.nih.gov/pubmed/33968895
http://dx.doi.org/10.3389/fchem.2021.628186
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author Luchini, Alessandra
Corucci, Giacomo
Chaithanya Batchu, Krishna
Laux, Valerie
Haertlein, Michael
Cristiglio, Viviana
Fragneto, Giovanna
author_facet Luchini, Alessandra
Corucci, Giacomo
Chaithanya Batchu, Krishna
Laux, Valerie
Haertlein, Michael
Cristiglio, Viviana
Fragneto, Giovanna
author_sort Luchini, Alessandra
collection PubMed
description Eukaryotic and prokaryotic cell membranes are difficult to characterize directly with biophysical methods. Membrane model systems, that include fewer molecular species, are therefore often used to reproduce their fundamental chemical and physical properties. In this context, natural lipid mixtures directly extracted from cells are a valuable resource to produce advanced models of biological membranes for biophysical investigations and for the development of drug testing platforms. In this study we focused on single phospholipid classes, i.e. Pichia pastoris phosphatidylcholine (PC) and Escherichia coli phosphatidylglycerol (PG) lipids. These lipids were characterized by a different distribution of their respective acyl chain lengths and number of unsaturations. We produced both hydrogenous and deuterated lipid mixtures. Neutron diffraction experiments at different relative humidities were performed to characterize multilayers from these lipids and investigate the impact of the acyl chain composition on the structural organization. The novelty of this work resides in the use of natural extracts with a single class head-group and a mixture of chain compositions coming from yeast or bacterial cells. The characterization of the PC and PG multilayers showed that, as a consequence of the heterogeneity of their acyl chain composition, different lamellar phases are formed.
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spelling pubmed-81040852021-05-08 Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction Luchini, Alessandra Corucci, Giacomo Chaithanya Batchu, Krishna Laux, Valerie Haertlein, Michael Cristiglio, Viviana Fragneto, Giovanna Front Chem Chemistry Eukaryotic and prokaryotic cell membranes are difficult to characterize directly with biophysical methods. Membrane model systems, that include fewer molecular species, are therefore often used to reproduce their fundamental chemical and physical properties. In this context, natural lipid mixtures directly extracted from cells are a valuable resource to produce advanced models of biological membranes for biophysical investigations and for the development of drug testing platforms. In this study we focused on single phospholipid classes, i.e. Pichia pastoris phosphatidylcholine (PC) and Escherichia coli phosphatidylglycerol (PG) lipids. These lipids were characterized by a different distribution of their respective acyl chain lengths and number of unsaturations. We produced both hydrogenous and deuterated lipid mixtures. Neutron diffraction experiments at different relative humidities were performed to characterize multilayers from these lipids and investigate the impact of the acyl chain composition on the structural organization. The novelty of this work resides in the use of natural extracts with a single class head-group and a mixture of chain compositions coming from yeast or bacterial cells. The characterization of the PC and PG multilayers showed that, as a consequence of the heterogeneity of their acyl chain composition, different lamellar phases are formed. Frontiers Media S.A. 2021-03-18 /pmc/articles/PMC8104085/ /pubmed/33968895 http://dx.doi.org/10.3389/fchem.2021.628186 Text en Copyright © 2021 Luchini, Corucci, Chaithanya Batchu, Laux, Haertlein, Cristiglio and Fragneto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Luchini, Alessandra
Corucci, Giacomo
Chaithanya Batchu, Krishna
Laux, Valerie
Haertlein, Michael
Cristiglio, Viviana
Fragneto, Giovanna
Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title_full Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title_fullStr Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title_full_unstemmed Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title_short Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction
title_sort structural characterization of natural yeast phosphatidylcholine and bacterial phosphatidylglycerol lipid multilayers by neutron diffraction
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104085/
https://www.ncbi.nlm.nih.gov/pubmed/33968895
http://dx.doi.org/10.3389/fchem.2021.628186
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