Cargando…
Umbilical cord miRNAs to predict neonatal early onset sepsis
OBJECTIVE: To determine if miRNA (miR) expression in umbilical cord blood and umbilical cord tissue differs between neonates with early onset sepsis (EOS) versus neonates without true infection. METHODS: Retrospective case-control study design of human patients with EOS (n = 8), presumed sepsis (N =...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104380/ https://www.ncbi.nlm.nih.gov/pubmed/33961620 http://dx.doi.org/10.1371/journal.pone.0249548 |
_version_ | 1783689483231690752 |
---|---|
author | Ernst, Linda M. Mithal, Leena B. Mestan, Karen Wang, Vivien Mangold, Kathy A. Freedman, Alexa Das, Sanchita |
author_facet | Ernst, Linda M. Mithal, Leena B. Mestan, Karen Wang, Vivien Mangold, Kathy A. Freedman, Alexa Das, Sanchita |
author_sort | Ernst, Linda M. |
collection | PubMed |
description | OBJECTIVE: To determine if miRNA (miR) expression in umbilical cord blood and umbilical cord tissue differs between neonates with early onset sepsis (EOS) versus neonates without true infection. METHODS: Retrospective case-control study design of human patients with EOS (n = 8), presumed sepsis (N = 12) and non-infected control patients (N = 21). Differential expression of >300 miRs was examined using the MIHS-3001ZE-miScript miRNA PCR Array Human miFinder 384HC. Expression levels of miRs were normalized using the global Ct mean of expressed miR and compared between groups. Data analysis was performed using GeneGlobe data analysis software. Ratios of over and under-expressed miRs were calculated and compared between groups using receiver operating characteristic (ROC) curves. RESULTS: Both umbilical cord plasma and umbilical cord tissue revealed several miRs with differential expression with little overlap between the two specimen types. The most overexpressed miR in plasma of EOS patients was miR-211-5p and the most overexpressed in EOS cord tissue was miR-223-5p. ROC curves comparing the ratios of over and under-expressed miRs for EOS patients and controls resulted in an area under the curve of 0.787 for cord plasma (miR-211-5p/miR-142-3p) and 0.988 for umbilical cord tissue (miR-223-5p/miR-22-3p), indicating good discrimination. CONCLUSIONS: miRs show differential expression in EOS versus non-infected controls and presumed sepsis. A ratio of over and under-expressed miRs can provide a potentially sensitive and specific diagnostic test for EOS. |
format | Online Article Text |
id | pubmed-8104380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81043802021-05-18 Umbilical cord miRNAs to predict neonatal early onset sepsis Ernst, Linda M. Mithal, Leena B. Mestan, Karen Wang, Vivien Mangold, Kathy A. Freedman, Alexa Das, Sanchita PLoS One Research Article OBJECTIVE: To determine if miRNA (miR) expression in umbilical cord blood and umbilical cord tissue differs between neonates with early onset sepsis (EOS) versus neonates without true infection. METHODS: Retrospective case-control study design of human patients with EOS (n = 8), presumed sepsis (N = 12) and non-infected control patients (N = 21). Differential expression of >300 miRs was examined using the MIHS-3001ZE-miScript miRNA PCR Array Human miFinder 384HC. Expression levels of miRs were normalized using the global Ct mean of expressed miR and compared between groups. Data analysis was performed using GeneGlobe data analysis software. Ratios of over and under-expressed miRs were calculated and compared between groups using receiver operating characteristic (ROC) curves. RESULTS: Both umbilical cord plasma and umbilical cord tissue revealed several miRs with differential expression with little overlap between the two specimen types. The most overexpressed miR in plasma of EOS patients was miR-211-5p and the most overexpressed in EOS cord tissue was miR-223-5p. ROC curves comparing the ratios of over and under-expressed miRs for EOS patients and controls resulted in an area under the curve of 0.787 for cord plasma (miR-211-5p/miR-142-3p) and 0.988 for umbilical cord tissue (miR-223-5p/miR-22-3p), indicating good discrimination. CONCLUSIONS: miRs show differential expression in EOS versus non-infected controls and presumed sepsis. A ratio of over and under-expressed miRs can provide a potentially sensitive and specific diagnostic test for EOS. Public Library of Science 2021-05-07 /pmc/articles/PMC8104380/ /pubmed/33961620 http://dx.doi.org/10.1371/journal.pone.0249548 Text en © 2021 Ernst et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ernst, Linda M. Mithal, Leena B. Mestan, Karen Wang, Vivien Mangold, Kathy A. Freedman, Alexa Das, Sanchita Umbilical cord miRNAs to predict neonatal early onset sepsis |
title | Umbilical cord miRNAs to predict neonatal early onset sepsis |
title_full | Umbilical cord miRNAs to predict neonatal early onset sepsis |
title_fullStr | Umbilical cord miRNAs to predict neonatal early onset sepsis |
title_full_unstemmed | Umbilical cord miRNAs to predict neonatal early onset sepsis |
title_short | Umbilical cord miRNAs to predict neonatal early onset sepsis |
title_sort | umbilical cord mirnas to predict neonatal early onset sepsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104380/ https://www.ncbi.nlm.nih.gov/pubmed/33961620 http://dx.doi.org/10.1371/journal.pone.0249548 |
work_keys_str_mv | AT ernstlindam umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT mithalleenab umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT mestankaren umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT wangvivien umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT mangoldkathya umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT freedmanalexa umbilicalcordmirnastopredictneonatalearlyonsetsepsis AT dassanchita umbilicalcordmirnastopredictneonatalearlyonsetsepsis |